Effects of Pseudomonas sp. OBA 2.4.1 on Growth and Tolerance to Cadmium Stress in Pisum sativum L

Liliya Khakimova; Olga Chubukova; Zilya Vershinina; Dilara Maslennikova

doi:10.3390/biotech12010005

Effects of Pseudomonas sp. OBA 2.4.1 on Growth and Tolerance to Cadmium Stress in Pisum sativum L

BioTech (Basel). 2023 Jan 3;12(1):5. doi: 10.3390/biotech12010005.

Authors

Liliya Khakimova^{1

2}, Olga Chubukova^{1

3}, Zilya Vershinina^{1

3}, Dilara Maslennikova^{1

3}

Affiliations

¹ Institute of Biochemistry and Genetics-Subdivision of the Ufa Federal Research Centre of the Russian Academy of Sciences, 450054 Ufa, Russia.
² Department of Fundamental and Applied Microbiology, Bashkir State Medical University (BSMU), 450008 Ufa, Russia.
³ Department of Molecular Technologis, Federal State Budgetary Educational Institution of Higher Education "Ufa State Petroleum Technological University" (USPTU), 450000 Ufa, Russia.

Abstract

Cadmium stress is a barrier to crop production, yield, quality, and sustainable agriculture. In the current study, we investigated the characteristics of bacterial strain Pseudomonas sp. OBA 2.4.1 under cadmium (CdCl₂) stress and its influence on Cd stresses in pea (Pisum sativum L.) seedlings. It was revealed that strain OBA 2.4.1 is tolerant of up to 2 mM CdCl₂, and seed treatment with the bacterium enhanced pea plant growth (length of seedlings) under 0.5 mM cadmium stress. This bacterial strain showed plant growth-promoting properties, including biofilm formation and siderophore activity. An important advantage of the studied strain OBA 2.4.1 is its ability to colonize the plant roots. Moreover, the inoculation with strain OBA 2.4.1 significantly reduced oxidative stress markers in pea seedlings under cadmium stress. These findings suggest that cadmium stress-tolerant strain OBA 2.4.1 could enhance pea plant growth by mitigating stress-caused damage, possibly providing a baseline and eco-friendly approach to address heavy metal stress for sustainable agriculture.

Keywords: PGPR; Pisum sativum L.; Pseudomonas sp.; biofilms; cadmium tolerance; growth stimulation; heavy metals (HMs); malondialdehyde.

Grants and funding

The publication was carried out within the framework of the state task of the Ministry of Science and Higher Education of the Russian Federation on the topic “Program for the Creation and Functioning of a Carbon Polygon in the Republic of Bashkortostan Eurasian Carbon Polygon for 2022-2023” (Publication number: FEUR-2022-0001).