Water striders of the subfamily Eotrechinae from the Phetchabun Mountain Range can be found at the margins of rock pools and puddles, and in hygropetric habitats including waterfalls. Twenty-two species in three genera are known from Thailand. In the Phetchabun Mountain Range, 12 species representing all three genera were recorded from over a decade of collections (2002-2012). This paper provides taxonomic, biological, and ecological information for taxa of the Phetchabun Mountain Range in Thailand and a taxonomic key to all known species from Thailand.