The Use of HRP in Decolorization of Reactive Dyes and Toxicological Evaluation of Their Products

Michelle Reis da Silva; Lívian Ribeiro Vasconcelos de Sá; Carlos Russo; Elita Scio; Viridiana Santana Ferreira-Leitão

doi:10.4061/2010/703824

The Use of HRP in Decolorization of Reactive Dyes and Toxicological Evaluation of Their Products

Enzyme Res. 2011 Jan 20:2010:703824. doi: 10.4061/2010/703824.

Authors

Michelle Reis da Silva¹, Lívian Ribeiro Vasconcelos de Sá, Carlos Russo, Elita Scio, Viridiana Santana Ferreira-Leitão

Affiliation

¹ Biocatalysis Laboratory, Catalysis Division, National Institute of Technology, Ministry of Science and Technology, Avenue Venezuela 82, Sala 302, 20081-312 Rio de Janeiro, RJ, Brazil.

Abstract

This work studied the potential use of horseradish peroxidase (HRP) in the decolorization of the following textile dyes: Drimarene Blue X-3LR (DMBLR), Drimarene Blue X-BLN (DMBBLN), Drimarene Rubinol X-3LR (DMR), and Drimarene Blue CL-R (RBBR). Dyes were individually tested in the reaction media containing 120 mg·L(-1), considering the following parameters: temperature (20-45°C), H(2)O(2) concentration (0-4.44 mmol·L(-1)), and reaction time (5 minutes, 1 and 24 h). The following conditions: 35°C, 0.55 mmol·L(-1), and 1h, provided the best set of results of color removal for DMBLR (99%), DMBBLN (77%), DMR (94%), and RBBR (97%). It should be mentioned that only 5 minutes of reaction was enough to obtain 96% of decolorization for DMBLR and RBBR. After the decolorization reactions of DMBLR, DMR, and RBBR, it was possible to observe the reduction of Artemia salina mortality and the no significant increase in toxicity for the products generated from DMBBLN.