Toxicity impact of hydrogen peroxide on the fate of zebrafish and antibiotic resistant bacteria

Hydrogen peroxide (H₂O₂) is applied in various environments. It could be present at concentrations ranging from nanomolar to micromolar in a water system. It is produced through pollutants and natural activities. Since few studies have been conducted about the impact of naturally produced H₂O₂ on aquatic organisms, the objective of the present study was to monitor changes in responses of aquatic model organisms such as zebrafish and antibiotic-resistant bacteria to different exogenous H₂O₂ exposure. Increases in exposure concentration and time induced decreases in the perception of zebrafish larvae (up to 69%) and movement of adult zebrafish (average speed, average acceleration, movement distance, and activity time) compared to the control (non-exposed group). In addition, as a function of H₂O₂ exposure concentration (0-100,000 nM) and time, up to 20-fold increase (p = 5.00*10^-6) of lipid peroxidation compared to control was observed. For microorganisms, biofilm, an indirect indicator of resistance to external stressors, was increased up to 68% and gene transfer was increased (p = 2.00*10^-6) by more than 30% after H₂O₂ exposure. These results imply that naturally generated H₂O₂ could adversely affect aquatic environment organisms and public health. Thus, more careful attention is needed for H₂O₂ production in an aquatic system.