Application of the SMALP technology to the isolation of GPCRs from low-yielding cell lines.
Tedesco D, Maj M, Malarczyk P, Cingolani A, Zaffagnini M, Wnorowski A, Czapiński J, Benelli T, Mazzoni R, Bartolini M, Jóźwiak K.
Tedesco D, et al.
Biochim Biophys Acta Biomembr. 2021 Sep 1;1863(9):183641. doi: 10.1016/j.bbamem.2021.183641. Epub 2021 May 10.
Biochim Biophys Acta Biomembr. 2021.
PMID: 33984320
Free article.
In-line combination of size-exclusion chromatography (SEC) with immobilized metal affinity chromatography (IMAC) allowed further improvement of the final rhbeta(2)AR yield by reducing the loss of SMALP-embedded GPCRs during the fractionation and purification of SMALPs. ...
In-line combination of size-exclusion chromatography (SEC) with immobilized metal affinity chromatography (IMAC) allowed further improvement …