Multi-Mycotoxin Occurrence in Dairy Cattle Feeds from the Gauteng Province of South Africa: A Pilot Study Using UHPLC-QTOF-MS/MS

The indispensable nature of toxigenic fungi and mycotoxins in agricultural systems is of worldwide concern, hence the need for surveillance studies to preserve public health. Thirteen dairy farms were surveyed and 40 dairy feeds of varying nature collected and analyzed for mycotoxins. Estimated levels of aflatoxins (AFs), fumonisin B₁ (FB₁), ochratoxin A (OTA), citrinin (CIT), zearalenone (ZEN), α-zearalenol (α-ZEL), β-zearalenol (β-ZEL), deoxynivalenol (DON), 3- and 15-acetyl-deoxynivalenol (ADONs), HT-2 toxin (HT-2), and beauvericin (BEA) were established using liquid chromatography-tandem mass spectrometry. Highest frequencies (40/40) were found for AFG₂ (range: <LOQ-116.1 ppb), α-ZEL (range: 0.98⁻13.24 ppb), and β-ZEL (range: 0.73⁻4.71 ppb), followed by AFB₂ at 37/40 (range: <LOQ-23.88 ppb), BEA at 36/40 (range: <LOQ-55.99 ppb), HT-2 at 35/40 (range: <LOQ-312.95 ppb), and FB₁ at 34/40 (range: <LOQ-1389.62 ppb). Apart from samples exceeding regulatory limits for total AFs in dairy feeds due to the high amounts of AFG₂ and AFB₂, levels of other mycotoxins were regarded as safe for dairy production in South Africa. This is the first-time the natural occurrence of the cold climate HT-2 in South African feeds was documented. Persistent co-occurrence of multiple mycotoxins across samples, however, may elicit synergistic and/or additive effects in hosts, hence raising concerns about their impacts and how such interactions may affect the dairy livestock sector.