Applications of CRISPR-Cas9 as an Advanced Genome Editing System in Life Sciences

Kamand Tavakoli; Alireza Pour-Aboughadareh; Farzad Kianersi; Peter Poczai; Alireza Etminan; Lia Shooshtari

doi:10.3390/biotech10030014

Applications of CRISPR-Cas9 as an Advanced Genome Editing System in Life Sciences

BioTech (Basel). 2021 Jul 6;10(3):14. doi: 10.3390/biotech10030014.

Authors

Kamand Tavakoli¹, Alireza Pour-Aboughadareh², Farzad Kianersi³, Peter Poczai⁴, Alireza Etminan⁵, Lia Shooshtari⁵

Affiliations

¹ Department of Medical Laboratory Science, Kermanshah Branch, Islamic Azad University, Kermanshah P.O. Box 6718997551, Iran.
² Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj P.O. Box 3158854119, Iran.
³ Department of Agronomy and Plant Breeding, Faculty of Agriculture, Bu-Ali Sina University, Hamedan P.O. Box 6517838695, Iran.
⁴ Botany Unit, Finnish Museum of Natural History, University of Helsinki, P.O. Box 7, FI-00014 Helsinki, Finland.
⁵ Department of Plant Breeding and Biotechnology, Kermanshah Branch, Islamic Azad University, Kermanshah P.O. Box 6718997551, Iran.

Abstract

Targeted nucleases are powerful genomic tools to precisely change the target genome of living cells, controlling functional genes with high exactness. The clustered regularly interspaced short palindromic repeats associated protein 9 (CRISPR-Cas9) genome editing system has been identified as one of the most useful biological tools in genetic engineering that is taken from adaptive immune strategies for bacteria. In recent years, this system has made significant progress and it has been widely used in genome editing to create gene knock-ins, knock-outs, and point mutations. This paper summarizes the application of this system in various biological sciences, including medicine, plant science, and animal breeding.

Keywords: CRISPR system; Cas proteins; agriculture; animal science; human disease.

Publication types

Review