HPLC separation of all aldopentoses and aldohexoses on an anion-exchange stationary phase prepared from polystyrene-based copolymer and diamine: the effect of NaOH eluent concentration

Molecules. 2011 Jul 14;16(7):5905-15. doi: 10.3390/molecules16075905.

Abstract

To investigate the separations of all aldopentoses (ribose, arabinose, xylose and lyxose) and aldohexoses (glucose, galactose, allose, altrose, mannose, gulose, idose and talose) on the D₆ stationary phase prepared by the reaction of chloromethylated styrene-divinylbenzene copolymer and N,N,N',N'-tetramethyl-1,6-diaminohexane, we examined the effect of varying the concentration of the NaOH eluent on the elution orders. Separations of these aldoses were achieved using a 20 mM NaOH eluent. The elution behaviors of the aldoses were probably due to not only the individual pK(a) values, but also the chemical structures of the cyclic aldoses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabinose / isolation & purification
  • Chromatography, High Pressure Liquid / methods*
  • Chromatography, Ion Exchange / methods*
  • Diamines / chemistry*
  • Galactose / isolation & purification
  • Glucose / isolation & purification
  • Hexoses / isolation & purification*
  • Mannose / isolation & purification
  • Pentoses / isolation & purification*
  • Polymers / chemistry*
  • Polystyrenes / chemistry*
  • Ribose / isolation & purification
  • Xylose / isolation & purification

Substances

  • Diamines
  • Hexoses
  • Pentoses
  • Polymers
  • Polystyrenes
  • gulose
  • idose
  • allose
  • lyxose
  • Ribose
  • Xylose
  • Arabinose
  • Glucose
  • Mannose
  • Galactose