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Cloning, expression and characterization of NADP-dependent isocitrate dehydrogenase from Staphylococcus aureus.
Prasad UV, Vasu D, Kumar YN, Kumar PS, Yeswanth S, Swarupa V, Phaneendra BV, Chaudhary A, Sarma PV. Prasad UV, et al. Appl Biochem Biotechnol. 2013 Feb;169(3):862-9. doi: 10.1007/s12010-012-0027-8. Epub 2013 Jan 4. Appl Biochem Biotechnol. 2013. PMID: 23288593
The expression of IDH in the clone UVIDH1 was induced with 1 mM IPTG, and the recombinant IDH was purified by passing through nickel metal chelate column; the purified recombinant IDH showed a single band in SDS-PAGE with a molecular weight of 40 kDa; K(m) and V(max) for i …
The expression of IDH in the clone UVIDH1 was induced with 1 mM IPTG, and the recombinant IDH was purified by passing through nickel metal c …