Prevalence, risk-factors, and antimicrobial susceptibility profile of methicillin-resistant Staphylococcus aureus (MRSA) obtained from nares of patients and staff of Sokoto state-owned hospitals in Nigeria

Shuaibu Suleiman Adeiza; Josiah Ademola Onaolapo; Busayo Olalekan Olayinka

doi:10.3205/dgkh000360

Prevalence, risk-factors, and antimicrobial susceptibility profile of methicillin-resistant Staphylococcus aureus (MRSA) obtained from nares of patients and staff of Sokoto state-owned hospitals in Nigeria

GMS Hyg Infect Control. 2020 Oct 12:15:Doc25. doi: 10.3205/dgkh000360. eCollection 2020.

Authors

Shuaibu Suleiman Adeiza¹, Josiah Ademola Onaolapo¹, Busayo Olalekan Olayinka¹

Affiliation

¹ Department of Pharmaceutical Microbiology, Faculty of Pharmaceutical sciences, Ahmadu Bello University, Zaria, Kaduna, Nigeria.

Abstract
in English, German

Aim: The aim of the study was to determine the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) obtained from the nasal cavity of participants and investigate the antibiotic resistance profiles of the isolates from Sokoto state, Nigeria. Methods: Nasal swabs of both nares were obtained from 378 participants across three study centers within the six-month study period. The Staphylococcus aureus isolates recovered were characterized, and their resistance phenotype determined in conjunction with MRSA prevalence. Results: Phenotypic screening of isolates obtained in this study revealed a total of 131 (17.3%) coagulase-positive Staphylococci out of 756 samples. Of this number, there were 81 (61.8%) S. aureus, 36 (27.5%) Staphylococcus intermedius, 6 (4.5%) Staphylococcus hyicus, and 8 (6.1%) Staphylococcus schleiferi. Conclusion: This study found a prevalence of 61.8% and 46.9% of S. aureus and MRSA among the studied hospitals in Sokoto state, thus demonstrating that the nares of the hospital populace are not free from S. aureus and MRSA colonization.

Ziel: Ziel der Studie war es, die Prävalenz von Methicillin-resistenten Staphylococcus aureus (MRSA) aus der Nasenhöhle der Teilnehmer zu bestimmen und die Antibiotika-Resistenz-Profile der Isolate aus dem Bundesstaat Sokoto, Nigeria, zu untersuchen. Methode: Von 378 Teilnehmern aus drei Studienzentren wurden im Verlauf von 6 Monaten Abstriche aus beiden Nares entnommen. Die gewonnenen Staphylococcus aureus-Isolate wurden charakterisiert und ihr Resistenz-Phänotyp in Verbindung mit der MRSA-Prävalenz bestimmt. Ergebnisse: Das phänotypische Screening der Isolate ergab 131 Koagulase-positive Staphylokokken, davon 81 Staphylococcus aureus (61,8%), 36 Staphylococcus intermedius (27,5%), 6 Staphylococcus hyicus (4,5%) und 8 Staphylococcus schleiferi (6,1%). Schlussfolgerung: Die Studie zeigt eine relative Prävalenzrate von 61,8% und 46,9% von S. aureus und MRSA unter den untersuchten Krankenhäusern im Bundesstaat Sokoto, was darauf schließen lässt, dass die Nasen der Krankenhausbevölkerung nicht frei von S. aureus und MRSA-Kolonisation sind.

Keywords: Staphylococcus aureus; antimicrobial resistance; dendrogram; methicillin resistance; regression.

Abstract in English, German

Abstract
in English, German