Photo-cured hyaluronic acid-based hydrogels containing simvastatin as a bone tissue regeneration scaffold

Min Soo Bae; Dae Hyeok Yang; Jung Bok Lee; Dong Nyoung Heo; Yong-Dae Kwon; In Chan Youn; Kuiwon Choi; Jong Hyun Hong; Gye Tae Kim; Yong Suk Choi; Eui Hwan Hwang; Il Keun Kwon

doi:10.1016/j.biomaterials.2011.07.045

Photo-cured hyaluronic acid-based hydrogels containing simvastatin as a bone tissue regeneration scaffold

Biomaterials. 2011 Nov;32(32):8161-71. doi: 10.1016/j.biomaterials.2011.07.045. Epub 2011 Aug 6.

Authors

Min Soo Bae¹, Dae Hyeok Yang, Jung Bok Lee, Dong Nyoung Heo, Yong-Dae Kwon, In Chan Youn, Kuiwon Choi, Jong Hyun Hong, Gye Tae Kim, Yong Suk Choi, Eui Hwan Hwang, Il Keun Kwon

Affiliation

¹ Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea.

PMID: 21821281
DOI: 10.1016/j.biomaterials.2011.07.045

Abstract

We describe in this study the positive influences on in vitro and in vivo osteogenesis of photo-cured hyaluronic acid (HA) hydrogels loaded with simvastatin (SIM). Prior to loading SIM, we first characterized the HA hydrogels for their mechanical properties and swelling ratios. The results from this testing indicated that these two factors improved as the substitution degree of 2-aminoethyl methacrylate (AEMA) increased. MTT and live/dead assays showed that the HA hydrogels have good biocompatibility for use as scaffolds for bone tissue regeneration. Moreover, another MTT assay showed that the photo-cured HA hydrogels III fabricated with 30% AEMA (300 mg) conjugated HA (HA-AEMA iii) loaded with between 0.1 and 1 mg of SIM had a similar cytotoxicity as compared to the HA hydrogel III itself. The sustained release of SIM was observed to occur in the HA hydrogel III loaded with 1 mg of SIM. In vitro and in vivo experiments showed that the HA hydrogel III loaded with 1 mg of SIM had a significant influence on osteogenesis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bone Regeneration / drug effects*
Calcification, Physiologic / drug effects
Calcification, Physiologic / radiation effects
Cell Death / drug effects
Cell Death / radiation effects
Cell Line
Cell Proliferation / drug effects
Cell Proliferation / radiation effects
Cell Survival / drug effects
Cell Survival / radiation effects
Fibroblasts / cytology
Fibroblasts / drug effects
Fibroblasts / radiation effects
Hyaluronic Acid / chemistry*
Hydrogels / chemistry*
Kinetics
Light*
Methacrylates / chemical synthesis
Methacrylates / chemistry
Mice
Osteogenesis / drug effects
Osteogenesis / genetics
Osteogenesis / radiation effects
Polymerization / drug effects
Polymerization / radiation effects
RNA, Messenger / genetics
RNA, Messenger / metabolism
Rabbits
Reverse Transcriptase Polymerase Chain Reaction
Rheology / drug effects
Rheology / radiation effects
Simvastatin / pharmacology*
Spectroscopy, Fourier Transform Infrared
Surface Properties / drug effects
Surface Properties / radiation effects
Tissue Scaffolds / chemistry*

Substances

Hydrogels
Methacrylates
RNA, Messenger
2-aminoethylmethacrylate
Hyaluronic Acid
Simvastatin