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The SARS-CoV-2 RNA-protein interactome in infected human cells.
Schmidt N, Lareau CA, Keshishian H, Ganskih S, Schneider C, Hennig T, Melanson R, Werner S, Wei Y, Zimmer M, Ade J, Kirschner L, Zielinski S, Dölken L, Lander ES, Caliskan N, Fischer U, Vogel J, Carr SA, Bodem J, Munschauer M. Schmidt N, et al. Nat Microbiol. 2021 Mar;6(3):339-353. doi: 10.1038/s41564-020-00846-z. Epub 2020 Dec 21. Nat Microbiol. 2021. PMID: 33349665 Free PMC article.
Using RNA antisense purification and mass spectrometry, we identified up to 104 human proteins that directly and specifically bind to SARS-CoV-2 RNAs in infected human cells. We integrated the SARS-CoV-2 RNA interactome with changes in proteome abundance induced by viral i …
Using RNA antisense purification and mass spectrometry, we identified up to 104 human proteins that directly and specifically bind to …
Binding of gephyrin to microtubules is regulated by its phosphorylation at Ser270.
Zhou L, Kiss E, Demmig R, Kirsch J, Nawrotzki RA, Kuhse J. Zhou L, et al. Histochem Cell Biol. 2021 Jul;156(1):5-18. doi: 10.1007/s00418-021-01973-2. Epub 2021 Apr 1. Histochem Cell Biol. 2021. PMID: 33796945 Free PMC article.
Gephyrin is a multifunctional scaffolding protein anchoring glycine- and subtypes of GABA type A- receptors at inhibitory postsynaptic membrane specializations by binding to the microtubule (MT) and/or the actin cytoskeleton. However, the conditions under which gephyrin can bi
Gephyrin is a multifunctional scaffolding protein anchoring glycine- and subtypes of GABA type A- receptors at inhibitory postsynaptic membr …
Complex formation of APP with GABAB receptors links axonal trafficking to amyloidogenic processing.
Dinamarca MC, Raveh A, Schneider A, Fritzius T, Früh S, Rem PD, Stawarski M, Lalanne T, Turecek R, Choo M, Besseyrias V, Bildl W, Bentrop D, Staufenbiel M, Gassmann M, Fakler B, Schwenk J, Bettler B. Dinamarca MC, et al. Nat Commun. 2019 Mar 22;10(1):1331. doi: 10.1038/s41467-019-09164-3. Nat Commun. 2019. PMID: 30902970 Free PMC article.
We now show that sequence-related epitopes in APP, AJAP-1 and PIANP bind with nanomolar affinities to the N-terminal sushi-domain of presynaptic GBRs. ...
We now show that sequence-related epitopes in APP, AJAP-1 and PIANP bind with nanomolar affinities to the N-terminal sushi-domain of …
A purine-rich element in foamy virus pol regulates env splicing and gag/pol expression.
Moschall R, Denk S, Erkelenz S, Schenk C, Schaal H, Bodem J. Moschall R, et al. Retrovirology. 2017 Feb 6;14(1):10. doi: 10.1186/s12977-017-0337-6. Retrovirology. 2017. PMID: 28166800 Free PMC article.
RESULTS: Here, we analysed env splicing and demonstrate that the described C element composed of three GAA repeats known to bind SR proteins regulates env splicing, thus balancing the amount of gag/pol mRNAs. ...
RESULTS: Here, we analysed env splicing and demonstrate that the described C element composed of three GAA repeats known to bind SR p …
PET imaging of cardiac wound healing using a novel [68Ga]-labeled NGR probe in rat myocardial infarction.
Tillmanns J, Schneider M, Fraccarollo D, Schmitto JD, Länger F, Richter D, Bauersachs J, Samnick S. Tillmanns J, et al. Mol Imaging Biol. 2015 Feb;17(1):76-86. doi: 10.1007/s11307-014-0751-2. Epub 2014 Jul 11. Mol Imaging Biol. 2015. PMID: 25011975
PURPOSE: Peptides containing the asparagine-glycine-arginine (NGR) motif bind to aminopeptidase N (CD13), which is expressed on inflammatory cells, endothelial cells, and fibroblasts. ...
PURPOSE: Peptides containing the asparagine-glycine-arginine (NGR) motif bind to aminopeptidase N (CD13), which is expressed on infla …
The AAA-ATPase Rea1 drives removal of biogenesis factors during multiple stages of 60S ribosome assembly.
Bassler J, Kallas M, Pertschy B, Ulbrich C, Thoms M, Hurt E. Bassler J, et al. Mol Cell. 2010 Jun 11;38(5):712-21. doi: 10.1016/j.molcel.2010.05.024. Mol Cell. 2010. PMID: 20542003 Free PMC article.
The AAA(+)-ATPase Rea1 removes the ribosome biogenesis factor Rsa4 from pre-60S ribosomal subunits in the nucleoplasm to drive nuclear export of the subunit. To do this, Rea1 utilizes a MIDAS domain to bind a conserved motif in Rsa4. Here, we show that the Rea1 MIDAS domai …
The AAA(+)-ATPase Rea1 removes the ribosome biogenesis factor Rsa4 from pre-60S ribosomal subunits in the nucleoplasm to drive nuclear expor …
Solution structure of the PsIAA4 oligomerization domain reveals interaction modes for transcription factors in early auxin response.
Dinesh DC, Kovermann M, Gopalswamy M, Hellmuth A, Calderón Villalobos LI, Lilie H, Balbach J, Abel S. Dinesh DC, et al. Proc Natl Acad Sci U S A. 2015 May 12;112(19):6230-5. doi: 10.1073/pnas.1424077112. Epub 2015 Apr 27. Proc Natl Acad Sci U S A. 2015. PMID: 25918389 Free PMC article.
The plant hormone auxin activates primary response genes by facilitating proteolytic removal of auxin/indole-3-acetic acid (AUX/IAA)-inducible repressors, which directly bind to transcriptional auxin response factors (ARF). Most AUX/IAA and ARF proteins share highly conser …
The plant hormone auxin activates primary response genes by facilitating proteolytic removal of auxin/indole-3-acetic acid (AUX/IAA)-inducib …