Boreal peatlands represent a large global carbon pool. The relationships between carbon mineralization, soil temperature and moisture in the permafrost peatlands of the Great Hing'an Mountains, China, were examined. The CO2 emissions were measured during laboratory incubations of samples from four sites under different temperatures (5, 10, 15, and 20 degrees C) and moisture contents (0%, 30%, 60%, 100% water holding capacity (WHC) and completely water saturated). Total carbon mineralization ranged from 15.51 to 112.92 mg C under the treatments for all sites. Carbon mineralization rates decreased with soil depth, increased with temperature, and reached the highest at 60% WHC at the same temperature. The calculated temperature coefficient (Q10) values ranged from 1.84 to 2.51 with the soil depths and moisture. However, the values were not significantly affected by soil moisture and depth for all sites due to the different peat properties (P > 0.05). We found that the carbon mineralization could be successfully predicted as a two-compartment function with temperature and moisture (R2 > 0.96) and total carbon mineralization was significantly affected by temperature and moisture (P < 0.05). Thus, temperature and moisture would play important roles in carbon mineralization of permafrost peatlands in the Great Hing'an Mountains, indicating that the permafrost peatlands would be sensitive to the environment change, and the permafrost peatlands would be potentially mineralized under future climate change.