A highly sensitive immunoassay using antibody-conjugated spherical mesoporous silica with immobilized enzymes

Chem Commun (Camb). 2014 Apr 7;50(27):3546-8. doi: 10.1039/c3cc48044e.

Abstract

A highly sensitive immunoassay was developed by using antibody-conjugated spherical mesoporous silica with immobilized enzymes. The higher ratio of enzyme/antibody than conventional ELISA improved both the sensitivity and dynamic range. Especially, the use of spherical mesoporous silica could achieve a limit of detection (LOD) with a sensitivity that is 20 times more than that of ELISA using amorphous silica.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Enzymes, Immobilized / chemistry*
  • Glucose Oxidase / chemistry*
  • Immunoassay
  • Immunoglobulin G / analysis*
  • Immunoglobulin G / chemistry
  • Limit of Detection
  • Silicon Dioxide / chemistry*

Substances

  • Enzymes, Immobilized
  • Immunoglobulin G
  • Silicon Dioxide
  • Glucose Oxidase