Assembly of bioactive multilayered nanocoatings on pancreatic islet cells: incorporation of α1-antitrypsin into the coatings

Chem Commun (Camb). 2015 Jul 7;51(53):10652-5. doi: 10.1039/c5cc02570b. Epub 2015 Jun 5.

Abstract

A spontaneous multilayer deposition approach for presenting therapeutic proteins onto pancreatic islet surfaces, using a heparin polyaldehyde and glycol chitosan alternating layering scheme, has been developed to enable the nanoscale engineering of a microenvironment for transplanted cells. The nanocoating incorporating α1-antitrypsin, an anti-inflammatory protein, exhibited effective anti-coagulant activities in vitro.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amines / chemistry
  • Animals
  • Cell Survival
  • Chitosan / chemistry
  • Fluorescein-5-isothiocyanate / chemistry
  • Heparin / chemistry
  • Insulin / metabolism
  • Islets of Langerhans / chemistry*
  • Islets of Langerhans / cytology
  • Islets of Langerhans / metabolism
  • Mice
  • Mice, Inbred ICR
  • Microscopy, Electron, Transmission
  • Nanostructures / chemistry*
  • alpha 1-Antitrypsin / chemistry*
  • alpha 1-Antitrypsin / metabolism

Substances

  • Amines
  • Insulin
  • alpha 1-Antitrypsin
  • glycol-chitosan
  • Heparin
  • Chitosan
  • Fluorescein-5-isothiocyanate