A novel biotransformation of 2-formyl-6-naphthoic acid to 2,6-naphthalene dicarboxylic acid by Pseudomonas sp. for the purification of crude 2,6-naphthalene dicarboxylic acid

Biotechnol Lett. 2008 Feb;30(2):329-33. doi: 10.1007/s10529-007-9545-9. Epub 2007 Oct 4.

Abstract

Crude 2,6-naphthalene dicarboxylic acid was purified by Pseudomonas sp. HN-72 which biotransformed the major impurity of 2-formyl-6-naphthoic acid into 2,6-naphthalene dicarboxylic acid. The biotransformation yield reached 100% when the reaction was performed at 40 degrees C for 1 h, in 200 ml KH(2)PO(4)/KOH buffer (50 mM, pH 8.0), with 0.2% (w/v) crude 2,6-naphthalene dicarboxylic acid and 2.5 mg dry cell wt/ml.

MeSH terms

  • Carboxylic Acids / chemistry*
  • Dicarboxylic Acids / chemistry*
  • Dicarboxylic Acids / isolation & purification*
  • Gas Chromatography-Mass Spectrometry
  • Naphthalenes / chemistry*
  • Naphthalenes / isolation & purification*
  • Pseudomonas / chemistry
  • Pseudomonas / isolation & purification*
  • Pseudomonas / metabolism*

Substances

  • 2-formyl-6-naphthoic acid
  • Carboxylic Acids
  • Dicarboxylic Acids
  • Naphthalenes
  • 2,6-naphthalenedicarboxylic acid