Biodegradation of oxadiazon by a soil isolated Pseudomonas fluorescens strain CG5: Implementation in an herbicide removal reactor and modelling

Water Res. 2006 Mar;40(6):1217-23. doi: 10.1016/j.watres.2006.01.010. Epub 2006 Mar 3.

Abstract

An oxadiazon-degrading bacterial, Pseudomonas strain CG5, was isolated from an agricultural contaminated soil. This strain CG5 was able to grow on 10mg of oxadiazon per l, yielding 5.18+/-0.2 mg of protein biomass mol(-1). GC-MS analyses of the metabolites from oxadiazon catabolism revealed its dehalogenation and degradation to form non-toxic end-products, cells were then immobilized by adsorption on a ceramic support to be used as biocatalysts in herbicide removal biofilm-reactor processes. Seventy-two per cent of the oxadiazon was removed, and the maximum specific substrate uptake rate was 10.63+/-0.5 microg h(-1) mg(-1) prot. A new mathematical model was developed to interpret and predict the behaviour of the bacteria and pollutants in a biofilm-reactor system, to consider biofilm structural and morphological properties.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biodegradation, Environmental
  • Bioreactors
  • Herbicides / metabolism*
  • Microscopy, Confocal
  • Models, Theoretical
  • Oxadiazoles / metabolism*
  • Pilot Projects
  • Pseudomonas fluorescens / metabolism*
  • Soil Microbiology*
  • Soil Pollutants / metabolism*

Substances

  • Herbicides
  • Oxadiazoles
  • Soil Pollutants
  • oxadiazon