Characterization of pathological stomach tissue using polarization-sensitive second harmonic generation microscopy

Hwanhee Jeon; MacAulay Harvey; Richard Cisek; Elisha Bennett; Danielle Tokarz

doi:10.1364/BOE.500335

Characterization of pathological stomach tissue using polarization-sensitive second harmonic generation microscopy

Biomed Opt Express. 2023 Sep 22;14(10):5376-5391. doi: 10.1364/BOE.500335. eCollection 2023 Oct 1.

Authors

Hwanhee Jeon¹, MacAulay Harvey¹, Richard Cisek¹, Elisha Bennett¹, Danielle Tokarz¹

Affiliation

¹ Department of Chemistry, Saint Mary's University, 923 Robie Street, Halifax, Nova Scotia, B3H 3C3, Canada.

Abstract

Alterations in collagen ultrastructure between human gastric adenocarcinoma and normal gastric tissue were investigated using polarization-resolved second harmonic generation (PSHG) microscopy. Cylindrical and trigonal symmetries were assumed to extract quantitative PSHG parameters, ρ, κ and S, from each image pixel. Statistically significant variations in these values were observed for gastric adenocarcinoma, indicating a higher disorder of collagen. Numerical focal volume simulations of crossing fibrils indicate increased S parameter is due to more intersecting collagen fibrils of varying diameters. These parameters were also able to distinguish between different grades of gastric adenocarcinoma indicating that PSHG may be useful for automated cancer diagnosis.