We studied the function of DYT1 promoter, found the important sectors controlling specific expression of DYT1 , and identified a new cis -element for further investigation of DYT1 upstream genes. DYT1 is a core regulatory gene for tapetum development in Arabidopsis thaliana. However, the mechanism leading to DYT1 tapetum-preferential expression is still unknown up to date. Here we employed promoter truncation and deletion assay to identify a 'CTCC' cis-element, which was essential for correct DYT1 expression within DYT1 promoter region. Through comparing truncated DYT1 promoter-driven GFP expression, the -481 to -513 bp region from the start point of transcription (SPT) of DYT1 was found indispensable for proper DYT1 expression. Further deletion assay around this region revealed that an approximate -468 bp 'CTCC' sequence deletion abolished normal DYT1 expression completely. Bioinformatics assay suggested that this 'CTCC' motif was potentially a novel DNA-recognition sequence, providing new clue for investigating relationship between DYT1 and its upstream genes.
Keywords: Arabidopsis; CTCC; Cis-element; DYT1; GCTGG; Tapetum.