Jump-seq: Genome-Wide Capture and Amplification of 5-Hydroxymethylcytosine Sites.
Hu L, Liu Y, Han S, Yang L, Cui X, Gao Y, Dai Q, Lu X, Kou X, Zhao Y, Sheng W, Gao S, He X, He C.
Hu L, et al.
J Am Chem Soc. 2019 Jun 5;141(22):8694-8697. doi: 10.1021/jacs.9b02512. Epub 2019 May 23.
J Am Chem Soc. 2019.
PMID: 31117646
Free PMC article.
The principle of this method is to label 5hmC by the 6- N3-glucose moiety and connect a hairpin DNA oligonucleotide carrying an alkyne group to the azide-modified 5hmC via Huisgen cycloaddition (click) chemistry. Primer extension starts from the hairpin motif to the modifi …
The principle of this method is to label 5hmC by the 6- N3-glucose moiety and connect a hairpin DNA oligonucleotide carrying an alkyne group …