Abstract
We developed an in vitro chromophore-solid phase peptide reaction assay (C-SPRA) using microbead-immobilized peptides and chromophores. Peptide-resins (microbeads) reacted with 14 representative chemicals to demonstrate the test's capacity to predict skin sensitization. C-SPRA enables accurate and high-throughput assessments of various chemicals, including poorly water-soluble sensitizers that are regarded as weakly potent by other methods.
MeSH terms
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Acrolein / analogs & derivatives
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Acrolein / chemistry
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Amino Acid Sequence
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Animals
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Benzothiazoles / chemistry
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Chromatography, High Pressure Liquid / methods*
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Chromatography, Reverse-Phase
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Coloring Agents / chemistry*
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Cysteine / chemistry
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Fluorescein-5-isothiocyanate / chemistry
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Immobilized Proteins / chemistry
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Lysine / chemistry
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Microspheres
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Peptides / chemistry*
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Skin / pathology
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Sulfhydryl Compounds / chemistry
Substances
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Benzothiazoles
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Coloring Agents
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Immobilized Proteins
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Peptides
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Sulfhydryl Compounds
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hexyl cinnamic aldehyde
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captax
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Acrolein
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Fluorescein-5-isothiocyanate
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Lysine
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Cysteine