Antibacterial Activity of Silver Nanoparticles (AgNP) Confined to Mesostructured, Silica-Based Calcium Phosphate Against Methicillin-Resistant Staphylococcus Aureus (MRSA)

Jung-Chang Kung; Wei-Hsun Wang; Chung-Lin Lee; Hao-Che Hsieh; Chi-Jen Shih

doi:10.3390/nano10071264

Antibacterial Activity of Silver Nanoparticles (AgNP) Confined to Mesostructured, Silica-Based Calcium Phosphate Against Methicillin-Resistant Staphylococcus Aureus (MRSA)

Nanomaterials (Basel). 2020 Jun 28;10(7):1264. doi: 10.3390/nano10071264.

Authors

Jung-Chang Kung^{1

2

3}, Wei-Hsun Wang^{4

5

6

7}, Chung-Lin Lee⁸, Hao-Che Hsieh⁸, Chi-Jen Shih^{3

8

9}

Affiliations

¹ School of Dentistry, College of Dental Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
² Department of Dentistry, Division of Family Dentistry, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan.
³ Drug Development and Value Creation Research Center, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
⁴ Department of Orthopedic Surgery, Changhua Christian Hospital, Changhua 500, Taiwan.
⁵ School of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
⁶ Department of Medical Imaging and Radiology, Shu-Zen Junior College of Medicine and Management, Kaohsiung 821, Taiwan.
⁷ Department of Golden-Ager Industry Management, Chaoyang University of Technology, Taichung 413, Taiwan.
⁸ Department of Fragrance and Cosmetic Science, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
⁹ Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan.

Abstract

Staphylococcus aureus, which is commonly found in hospitals, has become a major problem in infection control. In this study, Ag/80S bioactive ceramics used for enhanced antibacterial applications have been developed. An in vitro bioactivity test of the Ag/80S bioactive ceramic powders was performed in a phosphate-buffered saline (PBS). To explore the antibacterial activity of the Ag/80S bioactive ceramic powders, the Kirby-Bauer susceptibility test, the kinetics of microbial growth analysis and the colony-forming capacity assay were used to determine their minimum inhibitory concentration (MIC) against methicillin-resistant Staphylococcus aureus (MRSA). The results confirmed that the Ag/80S bioactive ceramic powders have antibacterial activity against MRSA (ATCC 33592) and MRSA (ATCC 49476).

Keywords: AgNP; antibacterial; mesostructured silica; minimum inhibitory concentration (MIC).

Abstract

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