Hydrogen Sulfide-Mediated Activation of O-Acetylserine (Thiol) Lyase and l/d-Cysteine Desulfhydrase Enhance Dehydration Tolerance in Eruca sativa Mill

Hydrogen sulfide (H₂S) has emerged as an important signaling molecule and plays a significant role during different environmental stresses in plants. The present work was carried out to explore the potential role of H₂S in reversal of dehydration stress-inhibited O-acetylserine (thiol) lyase (OAS-TL), l-cysteine desulfhydrase (LCD), and d-cysteine desulfhydrase (DCD) response in arugula (Eruca sativa Mill.) plants. Dehydration-stressed plants exhibited reduced water status and increased levels of hydrogen peroxide (H₂O₂) and superoxide (O₂^•-) content that increased membrane permeability and lipid peroxidation, and caused a reduction in chlorophyll content. However, H₂S donor sodium hydrosulfide (NaHS), at the rate of 2 mM, substantially reduced oxidative stress (lower H₂O₂ and O₂^•-) by upregulating activities of antioxidant enzymes (superoxide dismutase, peroxidase, and catalase) and increasing accumulation of osmolytes viz. proline and glycine betaine (GB). All these, together, resulted in reduced membrane permeability, lipid peroxidation, water loss, and improved hydration level of plants. The beneficial role of H₂S in the tolerance of plants to dehydration stress was traced with H₂S-mediated activation of carbonic anhydrase activity and enzyme involved in the biosynthesis of cysteine (Cys), such as OAS-TL. H₂S-treated plants showed maximum Cys content. The exogenous application of H₂S also induced the activity of LCD and DCD enzymes that assisted the plants to synthesize more H₂S from accumulated Cys. Therefore, an adequate concentration of H₂S was maintained, that improved the efficiency of plants to mitigate dehydration stress-induced alterations. The central role of H₂S in the reversal of dehydration stress-induced damage was evident with the use of the H₂S scavenger, hypotaurine.