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A 21S enzyme complex from HeLa cells that functions in simian virus 40 DNA replication in vitro.
Malkas LH, Hickey RJ, Li C, Pedersen N, Baril EF. Malkas LH, et al. Biochemistry. 1990 Jul 10;29(27):6362-74. doi: 10.1021/bi00479a004. Biochemistry. 1990. PMID: 2169868
In addition to the previously described 640-kDa multiprotein DNA polymerase alpha-primase complex [Vishwanatha et al. (1986) J. Biol. Chem. 261, 6619-6628], the enzyme complex also has associated topoisomerase I, DNA-dependent ATPase, RNase H, DNA ligase, a simian v …
In addition to the previously described 640-kDa multiprotein DNA polymerase alpha-primase complex [Vishwanatha et al. (1986) J. Biol. …
Characterization of the HeLa cell single-stranded DNA-dependent ATPase/DNA helicase II.
Vishwanatha JK, Tauer TJ, Rhode SL 3rd. Vishwanatha JK, et al. Mol Cell Biochem. 1995 May 24;146(2):121-6. doi: 10.1007/BF00944604. Mol Cell Biochem. 1995. PMID: 7565641
A single-stranded DNA-dependent ATPase activity, consisting of two subunits of 83 kDa (p90) and 68 kDa (p70), was previously purified from HeLa cells (Vishwanatha, J.K. and Baril, E.F. (1990) Biochem 29, 8753-8759). Homology of the two subunits of single-stranded DNA-depen …
A single-stranded DNA-dependent ATPase activity, consisting of two subunits of 83 kDa (p90) and 68 kDa (p70), was previously purified from H …
Characterization of Nα-Fmoc-protected dipeptide isomers by electrospray ionization tandem mass spectrometry (ESI-MS(n)): effect of protecting group on fragmentation of dipeptides.
Ramesh M, Raju B, Srinivas R, Sureshbabu VV, Vishwanatha TM, Hemantha HP. Ramesh M, et al. Rapid Commun Mass Spectrom. 2011 Jul 30;25(14):1949-58. doi: 10.1002/rcm.5076. Rapid Commun Mass Spectrom. 2011. PMID: 21698678
However, the peptides with Gly- at the N-terminus do not form b(1)(+) ions. The [M+H](+) ions of all the peptides undergo a McLafferty-type rearrangement followed by loss of CO(2) to form [M+H-Fmoc+H](+). ...Further, negative ion MS(3) CID has a …
However, the peptides with Gly- at the N-terminus do not form b(1)(+) ions. The [M+H](+) ions of all the peptides undergo a Mc …
4-Hydroxynonenal induces G2/M phase cell cycle arrest by activation of the ataxia telangiectasia mutated and Rad3-related protein (ATR)/checkpoint kinase 1 (Chk1) signaling pathway.
Chaudhary P, Sharma R, Sahu M, Vishwanatha JK, Awasthi S, Awasthi YC. Chaudhary P, et al. J Biol Chem. 2013 Jul 12;288(28):20532-46. doi: 10.1074/jbc.M113.467662. Epub 2013 Jun 3. J Biol Chem. 2013. PMID: 23733185 Free PMC article.
4-Hydroxynonenal (HNE) has been widely implicated in the mechanisms of oxidant-induced toxicity, but the detrimental effects of HNE associated with DNA damage or cell cycle arrest have not been thoroughly studied. Here we demonstrate for the first time that HNE caused G2/M …
4-Hydroxynonenal (HNE) has been widely implicated in the mechanisms of oxidant-induced toxicity, but the detrimental effects of HNE associat …
Regulation of angiotensin I-converting enzyme in cultured bovine bronchial epithelial cells.
Müns G, Vishwanatha JK, Rubinstein I. Müns G, et al. J Cell Biochem. 1993 Dec;53(4):352-9. doi: 10.1002/jcb.240530413. J Cell Biochem. 1993. PMID: 8300752
Western blot analysis of BBECs, tracheal and bronchial mucosal strips utilizing a cross-reacting rabbit anti-mouse ACE antibody, showed a faint 175 kDa band and additional strong 52 kDa and 47 kDa band. The mechanism of generation of the low M.W. bands is unknown. Our data …
Western blot analysis of BBECs, tracheal and bronchial mucosal strips utilizing a cross-reacting rabbit anti-mouse ACE antibody, showed a fa …
Production and characterization of a milk-clotting enzyme from Aspergillus oryzae MTCC 5341.
Vishwanatha KS, Appu Rao AG, Singh SA. Vishwanatha KS, et al. Appl Microbiol Biotechnol. 2010 Feb;85(6):1849-59. doi: 10.1007/s00253-009-2197-z. Epub 2009 Sep 2. Appl Microbiol Biotechnol. 2010. PMID: 19727708
Nearly 40,000 U/g bran of milk-clotting activity was present at the end of 120 h. The enzyme could be recovered by percolating the bran with 0.1 M sodium chloride for 60 min at 4 degrees Celsius. ...Affinity precipitation with alginate and subsequent elution with 0. …
Nearly 40,000 U/g bran of milk-clotting activity was present at the end of 120 h. The enzyme could be recovered by percolating the br …
Expression of 72 kDa and 92 kDa type IV collagenases from human giant-cell tumor of bone.
Rao VH, Bridge JA, Neff JR, Schaefer GB, Buehler BA, Vishwanatha JK, Pollock RE, Nicolson GL, Yamamoto M, Gokaslam ZL, et al. Rao VH, et al. Clin Exp Metastasis. 1995 Nov;13(6):420-6. doi: 10.1007/BF00118181. Clin Exp Metastasis. 1995. PMID: 7586800
In this study, we determined the enzymatic activity, content, and mRNA of both the 72 kDa (MMP-2) and 92 kDa (MMP-9) MMPs in primary cultures of human giant-cell tumor of bone (GCT) in vitro and in tissue extracts (in vivo). Gelatin zymography showed the presence of lytic bands a …
In this study, we determined the enzymatic activity, content, and mRNA of both the 72 kDa (MMP-2) and 92 kDa (MMP-9) MMPs in primary culture …
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