Abstract
Objectives:
We have used microarray technology to detect the effect of Guanxin No.2 decoction on gene expression in different areas of the myocardial infarcted heart of rats.
Methods:
Male Sprague-Dawley rats (180-200 g) were randomly divided into three groups: sham-operated; coronary artery ligation; and coronary artery ligation plus administration of Guanxin No.2 decoction (10.0 g raw materials/kg per day by gavage). The experiment was carried out on day seven after ligation.
Key findings:
We found that the gene expression using microarray technology showed many differences in the border infarcted left ventricular area compared with the remote noninfarcted left ventricular area after administration of Guanxin No.2 decoction.
Conclusions:
Guanxin No.2 decoction has a long history in treating ischaemic cardiomyopathy in China, but the molecular mechanism has been unclear. In this study we found that some important genes may have contributed to the cardioprotective effect of Guanxin No.2 decoction.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Benzaldehydes / chemistry
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Benzaldehydes / isolation & purification
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Benzoates / chemistry
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Benzoates / isolation & purification
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Benzofurans / chemistry
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Benzofurans / isolation & purification
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Bridged-Ring Compounds / chemistry
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Bridged-Ring Compounds / isolation & purification
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Cardiotonic Agents / chemistry
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Cardiotonic Agents / pharmacology
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Cardiotonic Agents / therapeutic use
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Carthamus tinctorius / chemistry
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Catechols / chemistry
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Catechols / isolation & purification
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Chalcone / analogs & derivatives
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Chalcone / chemistry
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Chalcone / isolation & purification
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Coumaric Acids / chemistry
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Coumaric Acids / isolation & purification
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Dalbergia / chemistry
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Drugs, Chinese Herbal / chemistry
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Drugs, Chinese Herbal / pharmacology*
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Drugs, Chinese Herbal / therapeutic use
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Gene Expression / drug effects*
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Gene Expression Profiling
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Glucosides / chemistry
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Glucosides / isolation & purification
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Heart Ventricles / drug effects
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Heart Ventricles / metabolism
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Hydroxybenzoates / chemistry
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Hydroxybenzoates / isolation & purification
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Lactates / chemistry
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Lactates / isolation & purification
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Ligusticum
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Male
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Monoterpenes
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Myocardial Infarction / drug therapy*
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Myocardial Infarction / genetics*
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Myocardial Infarction / metabolism
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Oligonucleotide Array Sequence Analysis / methods*
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Paeonia / chemistry
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Plant Extracts / chemistry
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Quinones / chemistry
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Quinones / isolation & purification
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Rats
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Rats, Sprague-Dawley
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Reverse Transcriptase Polymerase Chain Reaction
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Salvia miltiorrhiza / chemistry
Substances
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Benzaldehydes
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Benzoates
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Benzofurans
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Bridged-Ring Compounds
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Cardiotonic Agents
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Catechols
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Coumaric Acids
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Drugs, Chinese Herbal
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Glucosides
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Hydroxybenzoates
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Lactates
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Monoterpenes
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Plant Extracts
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Quinones
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guanxin No. 2
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hydroxysafflor yellow A
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peoniflorin
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protocatechuic acid
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3,4-dihydroxyphenyllactic acid
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protocatechualdehyde
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Chalcone
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ferulic acid
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salvianolic acid B
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chuangxiong extract