Background: The objective of this study is to detect HMGA2 expression in renal carcinoma to explore its relationship with clinicopathology and its significance in prognosis.
Methods: Expressions of HMGA2 mRNA and protein were detected in 50 renal carcinoma specimens, 50 corresponding adjacent normal kidney tissue samples and 40 renal benign tumour specimens via reverse transcription polymerase chain reaction and immunohistochemical assay. Expression analysis was performed along with clinical data analysis.
Results: The relative expression levels of HMGA2 mRNA in renal carcinoma, renal benign tumour tissues and adjacent normal renal tissues were 0.84±0.23, 0.19±0.06 and 0.08±0.04, respectively. HMGA2 protein positive rates were 68.0%, 7.5% and 2.0%, with a significant difference (P<0.05). HMGA2 expression was not significantly correlated with gender, age, tumour size and histological type (P>0.05), but was significantly correlated with TNM stages and lymph node metastasis (P<0.05).
Conclusions: The expressions of HMGA2 gene and protein in renal carcinoma were closely correlated with tumour formation, progression and metastasis. HMGA2 may become a powerful new pathological marker and prognostic factor for renal carcinoma.
Uvod: Cilj studije je da se otkrije ekspresija HMGA2 u karcinomu bubrega kako bi se istražio njen odnos sa kliničkom patologijom kao i značaj u prognozi.
Metode: Ekspresije mDNK i proteina HMGA2 određene su u 50 uzoraka karcinoma bubrega, 50 odgovarajućih uzoraka okolnog normalnog tkiva bubrega i 40 uzoraka benignih tumora bubrega putem reverzne transkripcije – lančane reakcije polimeraze i imunohistohemijskim testovima. Analiza ekspresije obavljena je zajedno sa analizom kliničkih podataka.
Rezultati: Relativni nivoi ekspresije mDNK HMGA2 u karcinomu bubrega, tkivima benignih bubrežnih tumora i tkivima okolnog normalnog bubrežnog tkiva bili su redom: 0,84±0,23, 0,19±0,06 i 0,08±0,04. Pozitivne stope proteina HMGA2 bile su 68,0%, 7,5% i 2,0%, uz postojanje značajne razlike (P<0,05). Ekspresija HMGA2 nije značajno korelisala sa polom, starošću, veličinom tumora i histološkim tipom (P>0,05), ali je bila u značajnoj korelaciji sa stupnjevima TNM i metastazama limfnih čvorova (P<0,05).
Zaključak: Ekspresije gena i proteina HMGA2 u karcinomu bubrega bile su u bliskoj korelaciji sa formiranjem, progresijom i metastazama tumora. HMGA2 bi mogao da postane moćan novi patološki marker i prognostički faktor za karcinom bubrega.
Keywords: RT-PCR; high mobility group A2; immunohistochemistry; renal carcinoma.