Detection of Enterobacterales resistant to polymyxins using Rapid Polymyxins NP test

Tanise Vendruscolo Dalmolin; Graziela Ávila Dias; Luiza Peres de Castro; Helena Ávila; Cibele Massotti Magagnin; Alexandre Prehn Zavascki; Daiana de Lima-Morales; Afonso Luís Barth

doi:10.1007/s42770-019-00053-x

Detection of Enterobacterales resistant to polymyxins using Rapid Polymyxins NP test

Braz J Microbiol. 2019 Apr;50(2):425-428. doi: 10.1007/s42770-019-00053-x. Epub 2019 Mar 11.

Authors

Affiliations

¹ Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
² Laboratório de Pesquisa em Resistência Bacteriana - LABRESIS, Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre (HCPA), 2350 Ramiro Barcelos St, Porto Alegre, RS, 90035-903, Brazil.
³ Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
⁴ Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil. albarth@hcpa.edu.br.
⁵ Laboratório de Pesquisa em Resistência Bacteriana - LABRESIS, Centro de Pesquisa Experimental, Hospital de Clínicas de Porto Alegre (HCPA), 2350 Ramiro Barcelos St, Porto Alegre, RS, 90035-903, Brazil. albarth@hcpa.edu.br.
⁶ Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil. albarth@hcpa.edu.br.

Abstract

Two hundred isolates of Enterobacterales were tested by Rapid Polymyxins NP for the detection of polymyxin resistance and compared to the reference test broth microdilution (BMD). The sensitivity and specificity of the NP test were 98% and the results are faster than the BMD, decreasing from approximately 24 to 2 h.

Keywords: Enterobacterales; Polymyxins; Rapid Polymyxins NP test; Susceptibility.

MeSH terms

Anti-Bacterial Agents / pharmacology*
Colistin / pharmacology*
Drug Resistance, Bacterial / genetics*
Enterobacteriaceae / drug effects*
Enterobacteriaceae / genetics*
Enterobacteriaceae / isolation & purification
Ethanolaminephosphotransferase / genetics
Humans
Microbial Sensitivity Tests
Polymyxin B / pharmacology*

Substances

Anti-Bacterial Agents
Ethanolaminephosphotransferase
Polymyxin B
Colistin