Biosensor Based on Tyrosinase Immobilized on Graphene-Decorated Gold Nanoparticle/Chitosan for Phenolic Detection in Aqueous

Fuzi Mohamed Fartas; Jaafar Abdullah; Nor Azah Yusof; Yusran Sulaiman; Mohd Izham Saiman

doi:10.3390/s17051132

Biosensor Based on Tyrosinase Immobilized on Graphene-Decorated Gold Nanoparticle/Chitosan for Phenolic Detection in Aqueous

Sensors (Basel). 2017 May 16;17(5):1132. doi: 10.3390/s17051132.

Authors

Fuzi Mohamed Fartas¹, Jaafar Abdullah^{2

3}, Nor Azah Yusof^{4

5}, Yusran Sulaiman^{6

7}, Mohd Izham Saiman⁸

Affiliations

¹ Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor D.E., Malaysia. fuzi2007@gmail.com.
² Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor D.E., Malaysia. jafar@upm.edu.my.
³ Institute of Advanced Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor D.E., Malaysia. jafar@upm.edu.my.
⁴ Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor D.E., Malaysia. azahy@upm.edu.my.
⁵ Institute of Advanced Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor D.E., Malaysia. azahy@upm.edu.my.
⁶ Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor D.E., Malaysia. yusran@upm.edu.my.
⁷ Institute of Advanced Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor D.E., Malaysia. yusran@upm.edu.my.
⁸ Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor D.E., Malaysia. mohdizham@upm.edu.my.

Abstract

In this research work, electrochemical biosensor was fabricated based on immobilization of tyrosinase onto graphene-decorated gold nanoparticle/chitosan (Gr-Au-Chit/Tyr) nanocomposite-modified screen-printed carbon electrode (SPCE) for the detection of phenolic compounds. The nanocomposite film was constructed via solution casting method. The electrocatalytic activity of the proposed biosensor for phenol detection was studied using differential pulse voltammetry (DPV) and cyclic voltammetry (CV). Experimental parameters such as pH buffer, enzyme concentration, ratio of Gr-Au-Chit, accumulation time and potential were optimized. The biosensor shows linearity towards phenol in the concentration range from 0.05 to 15 μM with sensitivity of 0.624 μA/μM and the limit of detection (LOD) of 0.016 μM (S/N = 3). The proposed sensor also depicts good reproducibility, selectivity and stability for at least one month. The biosensor was compared with high-performance liquid chromatography (HPLC) method for the detection of phenol spiked in real water samples and the result is in good agreement and comparable.

Keywords: electrochemical biosensor; graphene-gold nanocomposite; phenolic compounds; tyrosinase.