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Page 1
Showing results for fused tag
Search for Fusen Tang instead (2 results)
Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems.
Terpe K. Terpe K. Appl Microbiol Biotechnol. 2003 Jan;60(5):523-33. doi: 10.1007/s00253-002-1158-6. Epub 2002 Nov 7. Appl Microbiol Biotechnol. 2003. PMID: 12536251 Review.
Recombinant hybrids containing a polypeptide fusion partner, termed affinity tag, to facilitate the purification of the target polypeptides are widely used. ...This review gives an overview of the most frequently used and interesting systems: Arg-tag, calmodulin-bin …
Recombinant hybrids containing a polypeptide fusion partner, termed affinity tag, to facilitate the purification of the target polype …
The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins.
Schmidt TG, Skerra A. Schmidt TG, et al. Nat Protoc. 2007;2(6):1528-35. doi: 10.1038/nprot.2007.209. Nat Protoc. 2007. PMID: 17571060
The Strep-tag II is an eight-residue minimal peptide sequence (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys) that exhibits intrinsic affinity toward streptavidin and can be fused to recombinant proteins in various fashions. ...A high-affinity monoclonal antibody (StrepMAB-Immo) …
The Strep-tag II is an eight-residue minimal peptide sequence (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys) that exhibits intrinsic affinity towa …
A new metal affinity NCTR(25) tag as a better alternative to the His-tag for the expression of recombinant fused proteins.
Pan W, Wang Y, Wang N. Pan W, et al. Protein Expr Purif. 2019 Dec;164:105477. doi: 10.1016/j.pep.2019.105477. Epub 2019 Aug 13. Protein Expr Purif. 2019. PMID: 31419547
The NCTR(25)-tag and His-tag were separately fused to the transthyretin (TTR) protein, and the expression, affinity purification, refolding and stability of the two kinds of fusions were compared. ...Therefore, the native tag NCTR(25)-tag is a f …
The NCTR(25)-tag and His-tag were separately fused to the transthyretin (TTR) protein, and the expression, affinity pur …
Analyzing Protein Interactions by MAC-Tag Approaches.
Liu X, Salokas K, Keskitalo S, Martínez-Botía P, Varjosalo M. Liu X, et al. Methods Mol Biol. 2023;2690:281-297. doi: 10.1007/978-1-0716-3327-4_24. Methods Mol Biol. 2023. PMID: 37450155
BioID is one of the PL approaches, and it employs the expression of bait proteins fused to a nonspecific biotin ligase (BirA*), to induce in vivo biotinylation of proximal proteins. ...We therefore incorporate these tags into our system to develop MAC2-tag (containi …
BioID is one of the PL approaches, and it employs the expression of bait proteins fused to a nonspecific biotin ligase (BirA*), to in …
Transglutaminase 2 crosslinks the glutathione S-transferase tag, impeding protein-protein interactions of the fused protein.
Kim HJ, Lee JH, Lee KB, Shin JW, Kwon MA, Lee S, Jeong EM, Cho SY, Kim IG. Kim HJ, et al. Exp Mol Med. 2021 Jan;53(1):115-124. doi: 10.1038/s12276-020-00549-9. Epub 2021 Jan 13. Exp Mol Med. 2021. PMID: 33441971 Free PMC article.
Mutation analysis identified four glutamine residues in the GST tag as polyamination sites. TG2-mediated modification of the GST tag caused aggregate formation but did not affect its glutathione binding affinity. ...These results indicated that TG2 interferes with t …
Mutation analysis identified four glutamine residues in the GST tag as polyamination sites. TG2-mediated modification of the GST t …
Promoting Tag Removal of a MBP-Fused Integral Membrane Protein by TEV Protease.
Chen Y, Li Q, Yang J, Xie H. Chen Y, et al. Appl Biochem Biotechnol. 2017 Mar;181(3):939-947. doi: 10.1007/s12010-016-2260-z. Epub 2016 Sep 30. Appl Biochem Biotechnol. 2017. PMID: 27696139
Tag removal is a prerequisite issue for structural and functional analysis of affinity-purified membrane proteins. The present study took a MBP-fused membrane protein, MrpF, as a model to investigate the tag removal by TEV protease. ...
Tag removal is a prerequisite issue for structural and functional analysis of affinity-purified membrane proteins. The present study …
Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein.
Cabantous S, Terwilliger TC, Waldo GS. Cabantous S, et al. Nat Biotechnol. 2005 Jan;23(1):102-7. doi: 10.1038/nbt1044. Epub 2004 Dec 5. Nat Biotechnol. 2005. PMID: 15580262
Fluorogenic biarsenical FLaSH or ReASH substrates overcome many of these limitations but require a polycysteine tag motif, a reducing environment and cell transfection or permeabilization. ...We have engineered soluble, self-associating fragments of GFP that can be used to …
Fluorogenic biarsenical FLaSH or ReASH substrates overcome many of these limitations but require a polycysteine tag motif, a reducing …
Targeted Protein Depletion Using the Auxin-Inducible Degron 2 (AID2) System.
Saito Y, Kanemaki MT. Saito Y, et al. Curr Protoc. 2021 Aug;1(8):e219. doi: 10.1002/cpz1.219. Curr Protoc. 2021. PMID: 34370399
We recently established an improved system, called AID2, which involves expressing a mutant E3 ligase subunit, OsTIR1(F74G), and fusing a protein of interest to the mini-AID (mAID) tag, and that employs a new and more potent ligand, 5-phenyl-indole-3-acetic acid (5- …
We recently established an improved system, called AID2, which involves expressing a mutant E3 ligase subunit, OsTIR1(F74G), and fusing …
C-Terminally fused affinity Strep-tag II is removed by proteolysis from recombinant human erythropoietin expressed in transgenic tobacco plants.
Kittur FS, Lalgondar M, Hung CY, Sane DC, Xie J. Kittur FS, et al. Plant Cell Rep. 2015 Mar;34(3):507-16. doi: 10.1007/s00299-014-1730-4. Epub 2014 Dec 14. Plant Cell Rep. 2015. PMID: 25504272 Free PMC article.
C -terminally fused Strep -tag II is removed from rhuEPO expressed in tobacco plants. ...In this study, we investigated the stability of Strep-tag II tagged asialo-rhuEPO(P) expressed in tobacco plants to understand whether this fused tag is cle …
C -terminally fused Strep -tag II is removed from rhuEPO expressed in tobacco plants. ...In this study, we investigated the st …
Optimizing purification of the peripheral membrane protein FAM92A1 fused to a modified spidroin tag.
Wang Z, Mim C. Wang Z, et al. Protein Expr Purif. 2022 Jan;189:105992. doi: 10.1016/j.pep.2021.105992. Epub 2021 Oct 11. Protein Expr Purif. 2022. PMID: 34648955
Here, we present an optimized purification and expression strategy for dimeric FAM92A1. To our knowledge, we are the first to use the spidroin tag NT* to successfully purify a peripheral membrane protein. Our results show that NT* not only increases solubility but stabiliz …
Here, we present an optimized purification and expression strategy for dimeric FAM92A1. To our knowledge, we are the first to use the spidro …
2,597 results