In Vitro Cytotoxic Activity of Origanum vulgare L. on HCT-116 and MDA-MB-231 Cell Lines

Filip Grbović; Milan S Stanković; Milena Ćurčić; Nataša Đorđević; Dragana Šeklić; Marina Topuzović; Snežana Marković

doi:10.3390/plants2030371

In Vitro Cytotoxic Activity of Origanum vulgare L. on HCT-116 and MDA-MB-231 Cell Lines

Plants (Basel). 2013 Jun 25;2(3):371-8. doi: 10.3390/plants2030371.

Authors

Filip Grbović¹, Milan S Stanković², Milena Ćurčić¹, Nataša Đorđević³, Dragana Šeklić¹, Marina Topuzović¹, Snežana Marković¹

Affiliations

¹ Department of Biology and Ecology, Faculty of Science, University of Kragujevac, Str. Radoja Domanovića No. 12, 34000 Kragujevac, Republic of Serbia.
² Department of Biology and Ecology, Faculty of Science, University of Kragujevac, Str. Radoja Domanovića No. 12, 34000 Kragujevac, Republic of Serbia. mstankovic@kg.ac.rs.
³ Department of Biomedical Sciences, State University of Novi Pazar, Vuka Karadzica, 36300 Novi Pazar, Republic of Serbia.

Abstract

In the present investigation, we examined the cytotoxic effect of methanolic extract from Origanum vulgare on HCT-116 and MDA-MB-231 cell line in vitro. In order to determine the cytotoxic effects we used an MTT viability assay. The results showed that cell growth is significantly lower in extract treated cells compared to untreated control. The effect of inhibition of cell growth was higher in the treatment of HCT-116 cell line than in MDA-MB-231. Based on the results it is determined that O. vulgare is a significant source of biologically active substances that have cytotoxic and antiproliferative activity in vitro.

Keywords: HCT-116; MDA-MB-231; Origanum vulgare L.; antiproliferative activity.