Determination of chemical components of the endemic species Allium turcicum L. plant extract by LC-MS/MS and evaluation of medicinal potentials

Polat İpek; Mehmet Nuri Atalar; Ayşe Baran; Mehmet Fırat Baran; Mohammad Mehdi Ommati; Musa Karadag; Murat Zor; Aziz Eftekhari; Mehmet Hakkı Alma; Khaled Zoroufchi Benis; Fidan Nuriyeva; Rovshan Khalilov

doi:10.1016/j.heliyon.2024.e27386

Determination of chemical components of the endemic species Allium turcicum L. plant extract by LC-MS/MS and evaluation of medicinal potentials

Heliyon. 2024 Mar 13;10(6):e27386. doi: 10.1016/j.heliyon.2024.e27386. eCollection 2024 Mar 30.

Authors

Polat İpek¹, Mehmet Nuri Atalar², Ayşe Baran^{3

4}, Mehmet Fırat Baran⁵, Mohammad Mehdi Ommati⁶, Musa Karadag⁷, Murat Zor⁸, Aziz Eftekhari^{9

10

11}, Mehmet Hakkı Alma⁷, Khaled Zoroufchi Benis¹², Fidan Nuriyeva^{13

14}, Rovshan Khalilov^{15

10}

Affiliations

¹ Department of Physiology, Dicle University, Diyarbakir, Türkiye.
² Department of Nutrition and Dietetic, Faculty of Health Sciences, Iğdır University, Iğdır, Türkiye.
³ Department of Biology, Graduate Education Institute, Mardin Artuklu University, Mardin, Türkiye.
⁴ Malatya Turgut Özal University, Malatya, Türkiye.
⁵ Department of Food Technology, Vocational School of Technical Sciences, Batman University, Batman, Türkiye.
⁶ Henan Key Laboratory of Environmental and Animal Product Safety, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, 471000, China.
⁷ Research Application Laboratory and Research Center (ALUM), Iğdır University, Iğdır, Turkiye.
⁸ Department of Pharmacognosy, Fenerbahçe University, Ataşehir, İstanbul, Türkiye.
⁹ Department of Biochemistry, Faculty of Science, Ege University, Izmir 35040, Türkiye.
¹⁰ Nanotechnology and Biochemical Toxicology (NBT) center, Azerbaijan State University of Economics (UNEC), Baku AZ1001, Azerbaijan.
¹¹ Research Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz 51665118, Iran.
¹² Department of Process Engineering and Applied Science, Dalhousie University, Halifax, NS, Canada.
¹³ Department of Computer Science, Faculty of Science, Dokuz Eylul University, Izmir, Türkiye.
¹⁴ Laboratory of Recognition, Identification and Methods of Optimal Solutions, Institute of Control Systems, Baku, Azerbaijan.
¹⁵ Department of Biophysics and Biochemistry, Baku State University, Baku, Azerbaijan.

Abstract

The Allium turcicum L. (Zuzubak) plant as a cultivated vegetable have various health benefits and consumed as a food. Due to the shortcoming evidence in literature and the importance of this plant in folk medicine, in the present study, for the first time, we evaluated the bioactive profile of components (using LC-MS/MS), cytotoxicity, anticancer, antioxidant, and antibacterial prospectives of Zuzubak methanol extract. Reported results show that the extract is rich in bioactive compounds and has anticancer activity with breast cancer cells (MCF-7), human prostate cancer cells (DU-145), and Human osteosarcoma cancer Cell lines of (IC50) in dose dependent manner in the concentration range of 31.25 μg/mL and 2000 μg/mL for 24 and 48 h. Western blotting results determined that the extract significantly suppressed the growth of U2OS, MCF-7, and DU-145 cancer cells by down expression of Ang-1 (angiogenic protein) and Beclin-1 (autophagy protein) and overexpression of Bax (a proapoptotic protein). The oxidative stress indices showed a reduction in RPE-1 and MCF-7 cells and an upsurge in U2OS and DU-145 cells. Additionally, the antimicrobial assay showed suppression of the growth of various pathogenic microorganisms in 4.00-8.00 μg/concentrations of Zuzubak extract using the microdilution method. The phytochemicals identified showed promising anticancer, antioxidant effects, and antimicrobial properties, representing a valuable herbal source for drug development studies.

Keywords: Allium turcicum; Antimicrobial activity; Cytotoxic activity; Zuzubak.