Capillary ion chromatography-mass spectrometry for simultaneous determination of glucosylglycerol and sucrose in intracellular extracts of cyanobacteria

Yun Fa; Wenhui Liang; He Cui; Yangkai Duan; Menglong Yang; Jun Gao; Huizhou Liu

doi:10.1016/j.jchromb.2015.07.054

Capillary ion chromatography-mass spectrometry for simultaneous determination of glucosylglycerol and sucrose in intracellular extracts of cyanobacteria

J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Sep 15:1001:169-73. doi: 10.1016/j.jchromb.2015.07.054. Epub 2015 Jul 31.

Authors

Yun Fa¹, Wenhui Liang², He Cui³, Yangkai Duan⁴, Menglong Yang⁵, Jun Gao⁵, Huizhou Liu⁵

Affiliations

¹ Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao 266101, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: fayun@qibebt.ac.cn.
² Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao 266101, China; College of Food Science and Engineering, Shandong Agricultural University, Taian 271018, China.
³ Technical Center of Shandong Entry-Exit Inspection and Quarantine Bureau, No. 70 Qutangxia Road, Qingdao 266002, China.
⁴ University of Chinese Academy of Sciences, Beijing 100049, China.
⁵ Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao 266101, China.

PMID: 26279008
DOI: 10.1016/j.jchromb.2015.07.054

Abstract

A capillary ion chromatography-mass spectrometry (MS) method was proposed to determine glucosylglycerol (GG), sucrose, and five other carbohydrates. MS conditions and make-up flow parameters were optimized. This method is accurate and sensitive for simultaneous analysis of carbohydrates, with mean correlation coefficients of determination greater than 0.99, relative standard deviation of 0.91-2.81% for eight replicates, and average spiked recoveries of 97.3-104.9%. Limits of detection of sodium adduct were obtained with MS detection in selected ion mode for GG (0.006mg/L), sucrose (0.02mg/L), and other carbohydrates (0.03mg/L). This method was successfully applied to determine GG and sucrose in intracellular extracts of salt-stressed cyanobacteria.

Keywords: Capillary ion chromatography; Cyanobacteria; Glucosylglycerol; Mass spectrometry; sucrose.

Publication types

Research Support, Non-U.S. Gov't
Validation Study

MeSH terms

Chromatography, Liquid / methods*
Cyanobacteria / chemistry*
Glucosides / analysis*
Mass Spectrometry / methods*
Sucrose / analysis*

Substances

Glucosides
glucosylglycerol
Sucrose