Hydride bridge in [NiFe]-hydrogenase observed by nuclear resonance vibrational spectroscopy.
Ogata H, Krämer T, Wang H, Schilter D, Pelmenschikov V, van Gastel M, Neese F, Rauchfuss TB, Gee LB, Scott AD, Yoda Y, Tanaka Y, Lubitz W, Cramer SP.
Ogata H, et al.
Nat Commun. 2015 Aug 10;6:7890. doi: 10.1038/ncomms8890.
Nat Commun. 2015.
PMID: 26259066
Free PMC article.
Presented here is direct evidence for a hydride bridge in the active site of the (57)Fe-labelled fully reduced Ni-R form of Desulfovibrio vulgaris Miyazaki F [NiFe]-hydrogenase. A unique 'wagging' mode involving H(-) motion perpendicular to the Ni(mu-H)(57)Fe plane was stu …
Presented here is direct evidence for a hydride bridge in the active site of the (57)Fe-labelled fully reduced Ni-R form of Desulfovibrio vu …