The accuracy in determining sensible heat flux (H) of three Kipp and Zonen large aperture scintillometers (LAS) was evaluated with reference to an eddy covariance (EC) system over relatively flat and uniform grassland near Timpas (CO, USA). Other tests have revealed inherent variability between Kipp and Zonen LAS units and bias to overestimate H. Average H fluxes were compared between LAS units and between LAS and EC. Despite good correlation, inter-LAS biases in H were found between 6% and 13% in terms of the linear regression slope. Physical misalignment was observed to result in increased scatter and bias between H solutions of a well-aligned and poorly-aligned LAS unit. Comparison of LAS and EC H showed little bias for one LAS unit, while the other two units overestimated EC H by more than 10%. A detector alignment issue may have caused the inter-LAS variability, supported by the observation in this study of differing power requirements between LAS units. It is possible that the LAS physical misalignment may have caused edge-of-beam signal noise as well as vulnerability to signal noise from wind-induced vibrations, both having an impact on the solution of H. In addition, there were some uncertainties in the solutions of H from the LAS and EC instruments, including lack of energy balance closure with the EC unit. However, the results obtained do not show clear evidence of inherent bias for the Kipp and Zonen LAS to overestimate H as found in other studies.