Inhibition of Lipid Accumulation and Oxidation in Hepatocytes by Bioactive Bean Extracts

Dya Fita Dibwe; Emi Kitayama; Saki Oba; Nire Takeishi; Hitoshi Chiba; Shu-Ping Hui

doi:10.3390/antiox13050513

Inhibition of Lipid Accumulation and Oxidation in Hepatocytes by Bioactive Bean Extracts

Antioxidants (Basel). 2024 Apr 25;13(5):513. doi: 10.3390/antiox13050513.

Authors

Dya Fita Dibwe¹, Emi Kitayama², Saki Oba², Nire Takeishi², Hitoshi Chiba³, Shu-Ping Hui¹

Affiliations

¹ Faculty of Health Sciences, Hokkaido University, Kita-12, Nishi-5, Kita-Ku, Sapporo 060-0812, Japan.
² Graduate School of Health Sciences, Hokkaido University, Kita-12, Nishi-5, Kita-Ku, Sapporo 060-0812, Japan.
³ Department of Nutrition, Sapporo University of Health Sciences, Nakanuma Nishi-4-3-1-15, Higashi-Ku, Sapporo 007-0894, Japan.

Abstract

During our search for natural resources that can inhibit lipid droplet accumulation (LDA) and potentially prevent metabolic dysfunction-associated fatty liver disease (MAFLD) and its progressive stages, such as metabolic dysfunction-associated steatohepatitis (MASH), eight bean extracts (BE1-BE8) were tested for their ability to inhibit lipid accumulation and oxidation in hepatocytes. Substantial inhibitory effects on LDA with bean extracts (BEs) BE2, BE4, BE5, and BE8 were demonstrated. An advanced lipidomic approach was used to quantify the accumulation and inhibition of intracellular triacylglycerol (TAG) and its oxidized species, TAG hydroperoxide (TGOOH), in hepatocytes under fatty acid-loading conditions. The results show that the antioxidants BE2 and BE8 are potential candidates for regulating TAG and TGOOH accumulation in fatty acid-induced lipid droplets (LDs). This study suggests that bean-based foods inhibit LDs formation by decreasing intracellular lipids and lipid hydroperoxides in the hepatocytes. The metabolic profiling of BEs revealed that BE2 and BE8 contained polyphenolic compounds. These may be potential resources for the development of functional foods and drug discovery targeting MAFLD/MASH.

Keywords: bean extracts; functional foods; hepatosteatosis; hydroperoxides; neutral lipids; oxidative lipidomics; polyphenols; triacylglycerols.

Grants and funding

This research was supported by the Startup Research grant, Young Teacher Encouragement Grant (YTEG), and a research grant from the Faculty of Health Science at Hokkaido University (870411H) to D.F.D.