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Showing results for elisa hotchkiss
Search for Eliza Hotchkiss instead (1 results)
Adverse outcomes and an immunosuppressed endotype in septic patients with reduced IFN-γ ELISpot.
Barrios EL, Mazer MB, McGonagill PW, Bergmann CB, Goodman MD, Gould RW, Rao M, Polcz VE, Davis RJ, Del Toro DE, Dirain ML, Dram A, Hale LO, Heidarian M, Kim CY, Kucaba TA, Lanz JP, McCray AE, Meszaros S, Miles S, Nelson CR, Rocha IL, Silva EE, Ungaro RF, Walton AH, Xu J, Zeumer-Spataro L, Drewry AM, Liang M, Bible LE, Loftus TJ, Turnbull IR, Efron PA, Remy KE, Brakenridge SC, Badovinac VP, Griffith TS, Moldawer LL, Hotchkiss RS, Caldwell CC. Barrios EL, et al. JCI Insight. 2024 Jan 23;9(2):e175785. doi: 10.1172/jci.insight.175785. JCI Insight. 2024. PMID: 38100268 Free PMC article.
Here, we sought to determine whether functional immune responsiveness would yield improved precision.METHODSAn ex vivo whole-blood enzyme-linked immunosorbent spot (ELISpot) assay for cellular production of interferon gamma (IFN-gamma) was evaluated in …
Here, we sought to determine whether functional immune responsiveness would yield improved precision.METHODSAn ex vivo whole-blood enzyme …
Ca V 3.2 calcium channels contribute to trigeminal neuralgia.
Gambeta E, Gandini MA, Souza IA, Zamponi GW. Gambeta E, et al. Pain. 2022 Dec 1;163(12):2315-2325. doi: 10.1097/j.pain.0000000000002651. Epub 2022 Apr 20. Pain. 2022. PMID: 35467587
The effect of Z944 was absent in Ca V 3.2 -/- mice, indicating that Ca V 3.2 is the molecular target of the antihyperalgesic Z944 effect. Finally, enzyme-linked immunosorbent assay analysis revealed increased Ca V 3.2 channel expression in the spinal t …
The effect of Z944 was absent in Ca V 3.2 -/- mice, indicating that Ca V 3.2 is the molecular target of the antihyperalgesic Z944 effect. Fi …
Maternal Chronic Ultrasound Stress Provokes Immune Activation and Behavioral Deficits in the Offspring: A Mouse Model of Neurodevelopmental Pathology.
Pavlov D, Gorlova A, Haque A, Cavalcante C, Svirin E, Burova A, Grigorieva E, Sheveleva E, Malin D, Efimochkina S, Proshin A, Umriukhin A, Morozov S, Strekalova T. Pavlov D, et al. Int J Mol Sci. 2023 Jul 20;24(14):11712. doi: 10.3390/ijms241411712. Int J Mol Sci. 2023. PMID: 37511470 Free PMC article.
Additionally, the three-chamber sociability test and Barnes maze were used in the offspring groups. ELISA and qPCR were used to examine pro-inflammatory changes in the blood and hippocampus of adult females. ...
Additionally, the three-chamber sociability test and Barnes maze were used in the offspring groups. ELISA and qPCR were used to exami …
E-cigarette Aerosol Mixtures Inhibit Biomaterial-Induced Osseointegrative Cell Phenotypes.
Abaricia JO, Whitehead AJ, Kandalam S, Shah AH, Hotchkiss KM, Morandini L, Olivares-Navarrete R. Abaricia JO, et al. Materialia (Oxf). 2021 Dec;20:101241. doi: 10.1016/j.mtla.2021.101241. Epub 2021 Oct 8. Materialia (Oxf). 2021. PMID: 34778733 Free PMC article.
In MSCs, expression of osteogenic markers and inflammatory cytokines was measured by qPCR and ELISA, while alkaline phosphatase activity (ALP) was determined by colorimetric assay. ...
In MSCs, expression of osteogenic markers and inflammatory cytokines was measured by qPCR and ELISA, while alkaline phosphatase activ …
Pericytes as mediators of infiltration of macrophages in multiple sclerosis.
Kaushik DK, Bhattacharya A, Lozinski BM, Wee Yong V. Kaushik DK, et al. J Neuroinflammation. 2021 Dec 24;18(1):301. doi: 10.1186/s12974-021-02358-x. J Neuroinflammation. 2021. PMID: 34952601 Free PMC article.
These findings were validated using MS brain specimens, and further analysis of pericyte involvement in inflammation was carried out by culturing primary pericytes and macrophages. Multiplex ELISA, transmigration assay and real-time PCR were used to study the inflam …
These findings were validated using MS brain specimens, and further analysis of pericyte involvement in inflammation was carried out by cult …
Severe immunosuppression and not a cytokine storm characterizes COVID-19 infections.
Remy KE, Mazer M, Striker DA, Ellebedy AH, Walton AH, Unsinger J, Blood TM, Mudd PA, Yi DJ, Mannion DA, Osborne DF, Martin RS, Anand NJ, Bosanquet JP, Blood J, Drewry AM, Caldwell CC, Turnbull IR, Brakenridge SC, Moldwawer LL, Hotchkiss RS. Remy KE, et al. JCI Insight. 2020 Sep 3;5(17):e140329. doi: 10.1172/jci.insight.140329. JCI Insight. 2020. PMID: 32687484 Free PMC article.
Cadmium, an Environmental Contaminant, Exacerbates Alzheimer's Pathology in the Aged Mice's Brain.
Ali T, Khan A, Alam SI, Ahmad S, Ikram M, Park JS, Lee HJ, Kim MO. Ali T, et al. Front Aging Neurosci. 2021 Jun 24;13:650930. doi: 10.3389/fnagi.2021.650930. eCollection 2021. Front Aging Neurosci. 2021. PMID: 34248598 Free PMC article.
After behavioral studies, molecular/biochemical (Immunoblotting, ELISAs, ROS, LPO, and GSH assays) and morphological analyses were performed. ...
After behavioral studies, molecular/biochemical (Immunoblotting, ELISAs, ROS, LPO, and GSH assays) and morphological analyses were pe …
A Whole Blood Enzyme-Linked Immunospot Assay for Functional Immune Endotyping of Septic Patients.
Mazer MB, C Caldwell C, Hanson J, Mannion D, Turnbull IR, Drewry A, Osborne D, Walton A, Blood T, Moldawer LL, Brakenridge S, Remy KE, Hotchkiss RS. Mazer MB, et al. J Immunol. 2021 Jan 1;206(1):23-36. doi: 10.4049/jimmunol.2001088. Epub 2020 Nov 25. J Immunol. 2021. PMID: 33239423 Free PMC article.
We investigated the ability of a (to our knowledge) novel whole blood ELISpot assay and compared it with a more traditional ELISpot assay using PBMCs in sepsis. ...IL-7 restored ex vivo IFN-gamma production in septic patients. The whole blood ELISpot assay of …
We investigated the ability of a (to our knowledge) novel whole blood ELISpot assay and compared it with a more traditional ELISpot …
Temporal Changes in Innate and Adaptive Immunity During Sepsis as Determined by ELISpot.
Unsinger J, Osborne D, Walton AH, Han E, Sheets L, Mazer MB, Remy KE, Griffith TS, Rao M, Badovinac VP, Brackenridge SC, Turnbull I, Efron PA, Moldawer LL, Caldwell CC, Hotchkiss RS. Unsinger J, et al. bioRxiv [Preprint]. 2023 Dec 14:2023.12.14.571668. doi: 10.1101/2023.12.14.571668. bioRxiv. 2023. PMID: 38168302 Free PMC article. Preprint.
A key advantage of ELISpot is its excellent dynamic range enabling a more precise quantifiable assessment of host immunity. Herein, we tested the hypothesis on whether the ELISpot assay can detect dynamic changes in both innate and adaptive immunity as they often occur dur …
A key advantage of ELISpot is its excellent dynamic range enabling a more precise quantifiable assessment of host immunity. Herein, we teste …
Brain TNF drives post-inflammation depression-like behavior and persistent pain in experimental arthritis.
Lopes F, Vicentini FA, Cluny NL, Mathews AJ, Lee BH, Almishri WA, Griffin L, Gonçalves W, Pinho V, McKay DM, Hirota SA, Swain MG, Pittman QJ, Sharkey KA. Lopes F, et al. Brain Behav Immun. 2020 Oct;89:224-232. doi: 10.1016/j.bbi.2020.06.023. Epub 2020 Jun 24. Brain Behav Immun. 2020. PMID: 32592863
We measured joint pain using an automated dynamic plantar algesiometer and depression-like behavior with the tail suspension test. Cytokines were measured by Luminex assay and ELISA. TNF in the brain was blocked by intracerebroventricular injection of anti-TNF antib …
We measured joint pain using an automated dynamic plantar algesiometer and depression-like behavior with the tail suspension test. Cytokines …
51 results