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Evaluation of potential aging biomarkers in healthy individuals: telomerase, AGEs, GDF11/15, sirtuin 1, NAD+, NLRP3, DNA/RNA damage, and klotho.
Borsky P, Holmannova D, Andrys C, Kremlacek J, Fiala Z, Parova H, Rehacek V, Svadlakova T, Byma S, Kucera O, Borska L. Borsky P, et al. Biogerontology. 2023 Dec;24(6):937-955. doi: 10.1007/s10522-023-10054-x. Epub 2023 Jul 31. Biogerontology. 2023. PMID: 37523061 Free PMC article.
We included 169 healthy volunteers and divided them into groups according to age (under 35; 35-50; over 50) and sex (male, female; male and female under 35; 35-50, over 50). Markers were analyzed using commercial ELISA kits. We found differences in values depending on age …
We included 169 healthy volunteers and divided them into groups according to age (under 35; 35-50; over 50) and sex (male, female; male and …
Alarmins HMGB1, IL-33, S100A7, and S100A12 in Psoriasis Vulgaris.
Borsky P, Fiala Z, Andrys C, Beranek M, Hamakova K, Malkova A, Svadlakova T, Krejsek J, Palicka V, Borska L, Rehacek V. Borsky P, et al. Mediators Inflamm. 2020 Apr 15;2020:8465083. doi: 10.1155/2020/8465083. eCollection 2020. Mediators Inflamm. 2020. PMID: 32377165 Free PMC article.
METHODS: The serum levels of selected alarmins were measured in 63 psoriatic patients and 95 control individuals. The levels were assessed by the ELISA technique using commercial kits. The data were statistically processed with MedCalc version 19.0.5. ...
METHODS: The serum levels of selected alarmins were measured in 63 psoriatic patients and 95 control individuals. The levels were assessed b …
Metabolic Syndrome, Clusterin and Elafin in Patients with Psoriasis Vulgaris.
Holmannova D, Borsky P, Borska L, Andrys C, Hamakova K, Rehacek V, Svadlakova T, Malkova A, Beranek M, Palicka V, Krejsek J, Fiala Z. Holmannova D, et al. Int J Mol Sci. 2020 Aug 5;21(16):5617. doi: 10.3390/ijms21165617. Int J Mol Sci. 2020. PMID: 32764517 Free PMC article.
The aim of the present study is to contribute to the elucidation of pathophysiological links between psoriasis, its pro-inflammatory comorbidity metabolic syndrome (MetS), and the expression of clusterin and elafin, which are reflected in the pathophysiological "portfolio" of bot …
The aim of the present study is to contribute to the elucidation of pathophysiological links between psoriasis, its pro-inflammatory comorbi …
Telomere length, oxidative and epigenetic changes in blood DNA of patients with exacerbated psoriasis vulgaris.
Beranek M, Borsky P, Fiala Z, Andrys C, Hamakova K, Chmelarova M, Kovarikova H, Karas A, Kremlacek J, Palicka V, Borska L. Beranek M, et al. An Bras Dermatol. 2023 Jan-Feb;98(1):68-74. doi: 10.1016/j.abd.2022.01.008. Epub 2022 Oct 29. An Bras Dermatol. 2023. PMID: 36319514 Free PMC article.
Real-time PCR was used for the analysis of the telomere length. ELISA technique determined levels of 5-methylcytosine in blood cells' DNA. ...
Real-time PCR was used for the analysis of the telomere length. ELISA technique determined levels of 5-methylcytosine in blood cells' …
Goeckerman Regimen Reduces Alarmin Levels and PASI Score in Paediatric Patients with Psoriasis.
Holmannová D, Císařová B, Borský P, Fiala Z, Andrýs C, Hamaková K, Švadláková T, Krejsek J, Palička V, Kotingová L, Borská L. Holmannová D, et al. Acta Medica (Hradec Kralove). 2021;64(4):204-212. doi: 10.14712/18059694.2022.3. Acta Medica (Hradec Kralove). 2021. PMID: 35285442 Free article.
METHODS: The alarmin levels in sera of 19 paediatric patients with psoriasis were determined before and after GR using commercial ELISA kits. The Psoriasis area severity index (PASI) was used to determine the disease severity. ...
METHODS: The alarmin levels in sera of 19 paediatric patients with psoriasis were determined before and after GR using commercial ELISA
Aging in psoriasis vulgaris: female patients are epigenetically older than healthy controls.
Borsky P, Chmelarova M, Fiala Z, Hamakova K, Palicka V, Krejsek J, Andrys C, Kremlacek J, Rehacek V, Beranek M, Malkova A, Svadlakova T, Holmannova D, Borska L. Borsky P, et al. Immun Ageing. 2021 Mar 3;18(1):10. doi: 10.1186/s12979-021-00220-5. Immun Ageing. 2021. PMID: 33658053 Free PMC article.
The levels of endocan, vascular endothelial growth factor and interleukin-17 were analyzed using standardized ELISA methods. RESULTS: The difference between the epigenetic age and the chronological age of each individual subject did not increase with the increasing chronol …
The levels of endocan, vascular endothelial growth factor and interleukin-17 were analyzed using standardized ELISA methods. RESULTS: …
The Presence of Psoriasis, Metabolic Syndrome and Their Combination Increases the Serum Levels of CRP and CD5L but Not sCD200R1 and sTLR2 in Participants.
Holmannova D, Borsky P, Andrys C, Krejsek J, Cermakova E, Fiala Z, Hamakova K, Svadlakova T, Parova H, Rehacek V, Poctova G, Borska L. Holmannova D, et al. J Pers Med. 2022 Nov 28;12(12):1965. doi: 10.3390/jpm12121965. J Pers Med. 2022. PMID: 36556186 Free PMC article.
Material and methods: In the study, 64 controls and 43 patients with psoriasis with or without a metabolic syndrome were enrolled. The levels of selected markers were measured using ELISA kits. Results: CRP levels were significantly higher in psoriasis patients, especially …
Material and methods: In the study, 64 controls and 43 patients with psoriasis with or without a metabolic syndrome were enrolled. The level …
Significantly Altered Serum Levels of NAD, AGE, RAGE, CRP, and Elastin as Potential Biomarkers of Psoriasis and Aging-A Case-Control Study.
Karas A, Holmannova D, Borsky P, Fiala Z, Andrys C, Hamakova K, Svadlakova T, Palicka V, Krejsek J, Rehacek V, Esterkova M, Kovarikova H, Borska L. Karas A, et al. Biomedicines. 2022 May 13;10(5):1133. doi: 10.3390/biomedicines10051133. Biomedicines. 2022. PMID: 35625870 Free PMC article.
METHODS: The serum samples of 60 psoriatic patients and 100 controls were analysed, and the levels of four selected parameters (AGEs, RAGE, NAD, and elastin) were determined using commercial ELISA kits. Serum C-reactive protein was assayed using an immune-nephelometry meth …
METHODS: The serum samples of 60 psoriatic patients and 100 controls were analysed, and the levels of four selected parameters (AGEs, RAGE, …
Pregnancy-associated diamine oxidase originates from extravillous trophoblasts and is decreased in early-onset preeclampsia.
Velicky P, Windsperger K, Petroczi K, Pils S, Reiter B, Weiss T, Vondra S, Ristl R, Dekan S, Fiala C, Cantonwine DE, McElrath TF, Jilma B, Knöfler M, Boehm T, Pollheimer J. Velicky P, et al. Sci Rep. 2018 Apr 20;8(1):6342. doi: 10.1038/s41598-018-24652-0. Sci Rep. 2018. PMID: 29679053 Free PMC article.
Cultures of primary EVTs express and secret large amounts of bioactive DAO. ELISA measurements indicate a pregnancy-specific rise in maternal DAO plasma levels around gestational week (GW) 7 coinciding with vascular invasion of EVTs. ...
Cultures of primary EVTs express and secret large amounts of bioactive DAO. ELISA measurements indicate a pregnancy-specific rise in …
The Impact of Psoriasis and Metabolic Syndrome on the Systemic Inflammation and Oxidative Damage to Nucleic Acids.
Holmannova D, Borska L, Andrys C, Borsky P, Kremlacek J, Hamakova K, Rehacek V, Malkova A, Svadlakova T, Palicka V, Krejsek J, Fiala Z. Holmannova D, et al. J Immunol Res. 2020 May 20;2020:7352637. doi: 10.1155/2020/7352637. eCollection 2020. J Immunol Res. 2020. PMID: 32537470 Free PMC article.
The applicability of serum levels of calprotectin and ANGPTL8 for monitoring of the activity of psoriasis (diagnostic markers) is also evaluated. METHODS: Clinical examination (PASI score, MetS), enzyme-linked immunosorbent assay (ELISA), and En …
The applicability of serum levels of calprotectin and ANGPTL8 for monitoring of the activity of psoriasis (diagnostic markers) is also evalu …
32 results