Aneurysm-Specific miR-221 and miR-146a Participates in Human Thoracic and Abdominal Aortic Aneurysms

Premakumari Venkatesh; Julie Phillippi; Sasanka Chukkapalli; Mercedes Rivera-Kweh; Irina Velsko; Thomas Gleason; Paul VanRyzin; Seyed Hossein Aalaei-Andabili; Ravi Kiran Ghanta; Thomas Beaver; Edward Kar Leung Chan; Lakshmyya Kesavalu

doi:10.3390/ijms18040875

Aneurysm-Specific miR-221 and miR-146a Participates in Human Thoracic and Abdominal Aortic Aneurysms

Int J Mol Sci. 2017 Apr 20;18(4):875. doi: 10.3390/ijms18040875.

Affiliations

¹ Department of Periodontology, University of Florida, Gainesville, FL 32610, USA. vapg_1@yahoo.com.
² Department of Cardiothoracic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219, USA. phillippija@upmc.edu.
³ Department of Periodontology, University of Florida, Gainesville, FL 32610, USA. SChukkapalli@dental.ufl.edu.
⁴ Department of Periodontology, University of Florida, Gainesville, FL 32610, USA. mrivera4@ufl.edu.
⁵ Department of Periodontology, University of Florida, Gainesville, FL 32610, USA. irinavelsko@gmail.com.
⁶ Department of Cardiothoracic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219, USA. gleasontg@upmc.edu.
⁷ Department of Cardiothoracic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219, USA. paul@vanryzin.org.
⁸ Departments of Surgery, University of Florida, Gainesville, FL 32610, USA. hossein.aalaei@surgery.ufl.edu.
⁹ Michael E. Debakey Department of Surgery, Baylor College of Medicine, Houston, TX 77030, USA. Ravi.Ghanta@bcm.edu.
¹⁰ Departments of Surgery, University of Florida, Gainesville, FL 32610, USA. thomas.beaver@surgery.ufl.edu.
¹¹ Department of Oral Biology, University of Florida, Gainesville, FL 32610, USA. ECHAN@dental.ufl.edu.
¹² Department of Periodontology, University of Florida, Gainesville, FL 32610, USA. Kesavalu@dental.ufl.edu.
¹³ Department of Oral Biology, University of Florida, Gainesville, FL 32610, USA. Kesavalu@dental.ufl.edu.

Abstract

Altered microRNA expression is implicated in cardiovascular diseases. Our objective was to determine microRNA signatures in thoracic aortic aneurysms (TAAs) and abdominal aortic aneurysms (AAAs) compared with control non-aneurysmal aortic specimens. We evaluated the expression of fifteen selected microRNA in human TAA and AAA operative specimens compared to controls. We observed significant upregulation of miR-221 and downregulation of miR-1 and -133 in TAA specimens. In contrast, upregulation of miR-146a and downregulation of miR-145 and -331-3p were found only for AAA specimens. Upregulation of miR-126 and -486-5p and downregulation of miR-30c-2*, -155, and -204 were observed in specimens of TAAs and AAAs. The data reveal microRNA expression signatures unique to aneurysm location and common to both thoracic and abdominal pathologies. Thus, changes in miR-1, -29a, -133a, and -221 are involved in TAAs and miR-145, -146, and -331-3p impact AAAs. This work validates prior studies on microRNA expression in aneurysmal diseases.

Keywords: abdominal aortic aneurysm; microRNA; thoracic aortic aneurysm.

MeSH terms

Aged
Aortic Aneurysm, Abdominal / diagnosis
Aortic Aneurysm, Abdominal / genetics*
Aortic Aneurysm, Thoracic / diagnosis
Aortic Aneurysm, Thoracic / genetics*
Case-Control Studies
Cluster Analysis
Computational Biology / methods
Female
Gene Expression Profiling
Genetic Association Studies
Genetic Predisposition to Disease*
Humans
Male
MicroRNAs / genetics*
Middle Aged
Risk Factors
Transcriptome

Substances

MIRN146 microRNA, human
MIRN221 microRNA, human
MicroRNAs

Grants and funding

R01 HL109132/HL/NHLBI NIH HHS/United States