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Showing results for dicing wang
Search for Dicong Wang instead (1 results)
DEAD-box helicases modulate dicing body formation in Arabidopsis.
Li Q, Liu N, Liu Q, Zheng X, Lu L, Gao W, Liu Y, Liu Y, Zhang S, Wang Q, Pan J, Chen C, Mi Y, Yang M, Cheng X, Ren G, Yuan YW, Zhang X. Li Q, et al. Sci Adv. 2021 Apr 28;7(18):eabc6266. doi: 10.1126/sciadv.abc6266. Print 2021 Apr. Sci Adv. 2021. PMID: 33910901 Free PMC article.
Here, we investigated the roles of DEAD-box helicases in the formation and alteration of membraneless nuclear dicing bodies (D-bodies) in Arabidopsis thaliana We uncovered that RNA helicase 6 (RH6), RH8, and RH12 are previously unidentified D-body components. ...
Here, we investigated the roles of DEAD-box helicases in the formation and alteration of membraneless nuclear dicing bodies (D-bodies …
A SYBR Gold-based Label-free in vitro Dicing Assay.
Wang Q, Xue Y, Zhang L, Zhong Z, Feng S, Wang C, Xiao L, Yang Z, Harris JC, Wu Z, Zhai J, Yang M, Li S, Jacobsen SE, Du J. Wang Q, et al. Bio Protoc. 2022 Apr 5;12(7):e4382. doi: 10.21769/BioProtoc.4382. eCollection 2022 Apr 5. Bio Protoc. 2022. PMID: 35530519 Free PMC article.
To monitor the separation of the siRNA duplex with only 1-nt difference, we developed this protocol to evaluate the in vitro dicing activity of DCL3. The method can be applied for measuring the lengths of single-stranded RNA separated through denaturing urea polyacrylamide …
To monitor the separation of the siRNA duplex with only 1-nt difference, we developed this protocol to evaluate the in vitro dicing a …
Precision Layered Stealth Dicing of SiC Wafers by Ultrafast Lasers.
Yang B, Wang H, Peng S, Cao Q. Yang B, et al. Micromachines (Basel). 2022 Jun 27;13(7):1011. doi: 10.3390/mi13071011. Micromachines (Basel). 2022. PMID: 35888828 Free PMC article.
However, for SiC wafers with high hardness (Mohs hardness of 9.5), the diamond blade dicing suffers from problems such as debris contaminants and unnecessary thermal damage. In this work, a precision layered stealth dicing (PLSD) method by ultrafast lasers is propos …
However, for SiC wafers with high hardness (Mohs hardness of 9.5), the diamond blade dicing suffers from problems such as debris cont …
A DCL3 dicing code within Pol IV-RDR2 transcripts diversifies the siRNA pool guiding RNA-directed DNA methylation.
Loffer A, Singh J, Fukudome A, Mishra V, Wang F, Pikaard CS. Loffer A, et al. Elife. 2022 Jan 31;11:e73260. doi: 10.7554/eLife.73260. Elife. 2022. PMID: 35098919 Free PMC article.
We show that Pol IV's choice of initiating nucleotide, RDR2's initiation 1-2 nt internal to Pol IV transcript ends and RDR2's terminal transferase activity collectively yield a code that influences which precursor end is diced and whether 24 or 23 nt siRNAs are produced. B …
We show that Pol IV's choice of initiating nucleotide, RDR2's initiation 1-2 nt internal to Pol IV transcript ends and RDR2's terminal trans …
A high-efficiency gene silencing in plants using two-hit asymmetrical artificial MicroRNAs.
Teotia S, Wang X, Zhou N, Wang M, Liu H, Qin J, Han D, Li C, Li CE, Pan S, Tang H, Kang W, Zhang Z, Tang X, Peng T, Tang G. Teotia S, et al. Plant Biotechnol J. 2023 Sep;21(9):1799-1811. doi: 10.1111/pbi.14091. Epub 2023 Jul 1. Plant Biotechnol J. 2023. PMID: 37392408 Free PMC article.
MicroRNAs (miRNAs) are small non-coding RNA molecules that play a crucial role in gene regulation. They are produced through an enzyme-guided process called dicing and have an asymmetrical structure with two nucleotide overhangs at the 3' ends. ...
MicroRNAs (miRNAs) are small non-coding RNA molecules that play a crucial role in gene regulation. They are produced through an enzyme-guide …
Phase separation of SERRATE drives dicing body assembly and promotes miRNA processing in Arabidopsis.
Xie D, Chen M, Niu J, Wang L, Li Y, Fang X, Li P, Qi Y. Xie D, et al. Nat Cell Biol. 2021 Jan;23(1):32-39. doi: 10.1038/s41556-020-00606-5. Epub 2020 Dec 7. Nat Cell Biol. 2021. PMID: 33288888
MicroRNA (miRNA) production entails the step-wise processing of primary miRNAs (pri-miRNAs) into precursor miRNAs (pre-miRNAs) and miRNA/* duplexes by Dicing complexes containing DCL1, HYL1 and SE, which are localized in nuclear dicing bodies (D-bodies)(1,2). ...
MicroRNA (miRNA) production entails the step-wise processing of primary miRNAs (pri-miRNAs) into precursor miRNAs (pre-miRNAs) and miRNA/* d …
Mechanism of siRNA production by a plant Dicer-RNA complex in dicing-competent conformation.
Wang Q, Xue Y, Zhang L, Zhong Z, Feng S, Wang C, Xiao L, Yang Z, Harris CJ, Wu Z, Zhai J, Yang M, Li S, Jacobsen SE, Du J. Wang Q, et al. Science. 2021 Nov 26;374(6571):1152-1157. doi: 10.1126/science.abl4546. Epub 2021 Oct 14. Science. 2021. PMID: 34648373 Free PMC article.
Here, we determined the structure of a DCL3-pre-siRNA complex in an active dicing-competent state. The 5'-phosphorylated A1 of the guide strand and the 1-nt 3' overhang of the complementary strand are specifically recognized by a positively charged pocket and an aromatic c …
Here, we determined the structure of a DCL3-pre-siRNA complex in an active dicing-competent state. The 5'-phosphorylated A1 of the gu …
Good-Quality and High-Efficiency Dicing for Thick LiNbO(3) Wafers Using Picosecond Laser Pulses.
Lei M, Gao W, Li G, Wu X, Li B, Wang X, Wang J. Lei M, et al. Micromachines (Basel). 2019 Dec 31;11(1):51. doi: 10.3390/mi11010051. Micromachines (Basel). 2019. PMID: 31906043 Free PMC article.
By means of analysis of variance and analysis of relations between the characteristics, the optimal ps-laser-dicing parameter is obtained with good quality and high efficiency, which is applied to LiNbO(3) products. The result indicates that picosecond laser dicing …
By means of analysis of variance and analysis of relations between the characteristics, the optimal ps-laser-dicing parameter is obta …
Structural insights into dsRNA processing by Drosophila Dicer-2-Loqs-PD.
Su S, Wang J, Deng T, Yuan X, He J, Liu N, Li X, Huang Y, Wang HW, Ma J. Su S, et al. Nature. 2022 Jul;607(7918):399-406. doi: 10.1038/s41586-022-04911-x. Epub 2022 Jun 29. Nature. 2022. PMID: 35768513 Free PMC article.
Additional ATP-dependent conformational changes are required to form an active dicing state and precisely cleave the dsRNA into a 21 bp siRNA duplex as confirmed by the structure in the post-dicing state. ...
Additional ATP-dependent conformational changes are required to form an active dicing state and precisely cleave the dsRNA into a 21 …
62 results