Two pear glutathione S-transferases genes are regulated during fruit development and involved in response to salicylic acid, auxin, and glucose signaling

Hai-Yan Shi; Zheng-Hong Li; Yu-Xing Zhang; Liang Chen; Di-Ying Xiang; Yu-Feng Zhang

doi:10.1371/journal.pone.0089926

Two pear glutathione S-transferases genes are regulated during fruit development and involved in response to salicylic acid, auxin, and glucose signaling

PLoS One. 2014 Feb 25;9(2):e89926. doi: 10.1371/journal.pone.0089926. eCollection 2014.

Authors

Hai-Yan Shi¹, Zheng-Hong Li¹, Yu-Xing Zhang¹, Liang Chen², Di-Ying Xiang¹, Yu-Feng Zhang¹

Affiliations

¹ College of Horticulture, Agricultural University of Hebei, Baoding, China.
² Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture and Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, China.

Abstract

Two genes encoding putative glutathione S-transferase proteins were isolated from pear (Pyrus pyrifolia) and designated PpGST1 and PpGST2. The deduced PpGST1 and PpGST2 proteins contain conserved Glutathione S-transferase N-terminal domain (GST_N) and Glutathione S-transferase, C-terminal domain (GST_C). Using PCR amplification technique, the genomic clones corresponding to PpGST1 and PpGST2 were isolated and shown to contain two introns and a singal intron respectively with typical GT/AG boundaries defining the splice junctions. Phylogenetic analysis clearly demonstrated that PpGST1 belonged to Phi class of GST superfamilies and had high homology with apple MdGST, while PpGST2 was classified into the Tau class of GST superfamilies. The expression of PpGST1 and PpGST2 genes was developmentally regulated in fruit. Further study demonstrated that PpGST1 and PpGST2 expression was remarkably induced by glucose, salicylic acid (SA) and indole-3-aceticacid (IAA) treatments in pear fruit, and in diseased fruit. These data suggested that PpGST1 and PpGST2 might be involved in response to sugar, SA, and IAA signaling during fruit development of pear.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Base Sequence
Cluster Analysis
DNA Primers / genetics
Fruit / growth & development*
Fruit / metabolism
Gene Expression Regulation, Plant / drug effects
Gene Expression Regulation, Plant / physiology*
Gene Library
Glucose / pharmacology
Glutathione Transferase / classification
Glutathione Transferase / genetics*
Glutathione Transferase / metabolism*
Indoleacetic Acids / pharmacology
Molecular Sequence Data
Phylogeny*
Protein Structure, Tertiary / genetics
Pyrus / enzymology*
Real-Time Polymerase Chain Reaction
Salicylic Acid / pharmacology
Sequence Alignment
Sequence Analysis, DNA
Signal Transduction / physiology*

Substances

DNA Primers
Indoleacetic Acids
indoleacetic acid
Glutathione Transferase
Glucose
Salicylic Acid

Grants and funding

This work was supported by the Research Fund for the Doctoral Program of Higher Education of China (Grant No. 20121302120004), the Hebei Province Science Foundation of China (Grant No. C2013204051), and the National Natural Sciences Foundation of China (Grant No. 31301761). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of manuscript.