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D(2)O as an Imperfect Replacement for H(2)O: Problem or Opportunity for Protein Research?
Giubertoni G, Bonn M, Woutersen S. Giubertoni G, et al. J Phys Chem B. 2023 Sep 28;127(38):8086-8094. doi: 10.1021/acs.jpcb.3c04385. Epub 2023 Sep 18. J Phys Chem B. 2023. PMID: 37722111 Free PMC article. Review.
D(2)O is chemically equivalent to H(2)O, and the differences, particularly in hydrogen-bond strength, are often ignored. However, replacing solvent water with D(2)O can affect not only the kinetics but also the structure and stability of biomolecules. ...
D(2)O is chemically equivalent to H(2)O, and the differences, particularly in hydrogen-bond strength, are often ignored. However, rep
Molecular Origin of the Elastic State of Aqueous Hyaluronic Acid.
Giubertoni G, Burla F, Martinez-Torres C, Dutta B, Pletikapic G, Pelan E, Rezus YLA, Koenderink GH, Bakker HJ. Giubertoni G, et al. J Phys Chem B. 2019 Apr 11;123(14):3043-3049. doi: 10.1021/acs.jpcb.9b00982. Epub 2019 Mar 28. J Phys Chem B. 2019. PMID: 30888176 Free PMC article.
We find that the gelation originates from the enhanced formation of strong interchain connections, consisting of a double amide-COOH hydrogen bond and an N-D-COO(-) hydrogen bond on the adjacent sugars of the hyaluronan disaccharide unit. ...
We find that the gelation originates from the enhanced formation of strong interchain connections, consisting of a double amide-COOH hydroge …
Stress Echocardiography in Italian Echocardiographic Laboratories: A Survey of the Italian Society of Echocardiography and Cardiovascular Imaging.
Ciampi Q, Pepi M, Antonini-Canterin F, Barbieri A, Barchitta A, Faganello G, Miceli S, Parato VM, Tota A, Trocino G, Abbate M, Accadia M, Alemanni R, Angelini A, Anglano F, Anselmi M, Aquila I, Aramu S, Avogadri E, Azzaro G, Badano L, Balducci A, Ballocca F, Barbarossa A, Barbati G, Barletta V, Barone D, Becherini F, Benfari G, Beraldi M, Bergandi G, Bilardo G, Binno SM, Bolognesi M, Bongiovi S, Bragato RM, Braggion G, Brancaleoni R, Bursi F, Dessalvi CC, Cameli M, Canu A, Capitelli M, Capra ACM, Carbonara R, Carbone M, Carbonella M, Carrabba N, Casavecchia G, Casula M, Chesi E, Cicco S, Citro R, Cocchia R, Colombo BM, Colonna P, Conte M, Corrado G, Cortesi P, Cortigiani L, Costantino MF, Cozza F, Cucchini U, D'Angelo M, Da Ros S, D'Andrea F, D'Andrea A, D'Auria F, De Caridi G, De Feo S, De Matteis GM, De Vecchi S, Del Giudice C, Dell'Angela L, Paoli LD, Dentamaro I, Destefanis P, Di Bella G, Di Fulvio M, Di Gaetano R, Di Giannuario G, Di Gioia A, Di Martino LFM, Di Muro C, Di Nora C, Di Salvo G, Dodi C, Dogliani S, Donati F, Dottori M, Epifani G, Fabiani I, Ferrara F, Ferrara L, Ferrua S, Filice G, Fiorino M, Forno D, Garini A, Giarratana GA, Gigantino G, Giorgi M, Giubertoni E, … See abstract for full author list ➔ Ciampi Q, et al. J Cardiovasc Echogr. 2023 Jul-Sep;33(3):125-132. doi: 10.4103/jcecho.jcecho_48_23. Epub 2023 Nov 20. J Cardiovasc Echogr. 2023. PMID: 38161775 Free PMC article.
Labile assembly of a tardigrade protein induces biostasis.
Sanchez-Martinez S, Nguyen K, Biswas S, Nicholson V, Romanyuk AV, Ramirez J, Kc S, Akter A, Childs C, Meese EK, Usher ET, Ginell GM, Yu F, Gollub E, Malferrari M, Francia F, Venturoli G, Martin EW, Caporaletti F, Giubertoni G, Woutersen S, Sukenik S, Woolfson DN, Holehouse AS, Boothby TC. Sanchez-Martinez S, et al. Protein Sci. 2024 Apr;33(4):e4941. doi: 10.1002/pro.4941. Protein Sci. 2024. PMID: 38501490 Free PMC article.
Here we report a mechanism of fibrillization and gelation for CAHS D similar to that of intermediate filament assembly. We show that in vitro, gelation restricts molecular motion, immobilizing and protecting labile material from the harmful effects of drying. In vivo, we o …
Here we report a mechanism of fibrillization and gelation for CAHS D similar to that of intermediate filament assembly. We show that …
Organization and Activity of Italian Echocardiographic Laboratories: A Survey of the Italian Society of Echocardiography and Cardiovascular Imaging.
Ciampi Q, Pepi M, Antonini-Canterin F, Barbieri A, Barchitta A, Faganello G, Miceli S, Parato VM, Tota A, Trocino G, Abbate M, Accadia M, Alemanni R, Angelini A, Anglano F, Anselmi M, Aquila I, Aramu S, Avogadri E, Azzaro G, Badano L, Balducci A, Ballocca F, Barbarossa A, Barbati G, Barletta V, Barone D, Becherini F, Benfari G, Beraldi M, Bergandi G, Bilardo G, Binno SM, Bolognesi M, Bongiovi S, Bragato RM, Braggion G, Brancaleoni R, Bursi F, Dessalvi CC, Cameli M, Canu A, Capitelli M, Capra ACM, Carbonara R, Carbone M, Carbonella M, Carrabba N, Casavecchia G, Casula M, Chesi E, Cicco S, Citro R, Cocchia R, Colombo BM, Colonna P, Conte M, Corrado G, Cortesi P, Cortigiani L, Costantino MF, Cozza F, Cucchini U, D'Angelo M, Ros SD, D'Andrea F, D'Andrea A, D'Auria F, De Caridi G, De Feo S, De Matteis GM, De Vecchi S, Giudice CD, Dell'Angela L, Paoli LD, Dentamaro I, Destefanis P, Di Fulvio M, Di Gaetano R, Di Giannuario G, Di Gioia A, Di Martino LFM, Di Muro C, Di Nora C, Di Salvo G, Dodi C, Dogliani S, Donati F, Dottori M, Epifani G, Fabiani I, Ferrara F, Ferrara L, Ferrua S, Filice G, Fiorino M, Forno D, Garini A, Giarratana GA, Gigantino G, Giorgi M, Giubertoni E, Greco CA, Grigola… See abstract for full author list ➔ Ciampi Q, et al. J Cardiovasc Echogr. 2023 Jan-Mar;33(1):1-9. doi: 10.4103/jcecho.jcecho_16_23. Epub 2023 May 29. J Cardiovasc Echogr. 2023. PMID: 37426716 Free PMC article.
The use of magnetic susceptibility as a forensic search tool.
Pringle JK, Giubertoni M, Cassidy NJ, Wisniewski KD, Hansen JD, Linford NT, Daniels RM. Pringle JK, et al. Forensic Sci Int. 2015 Jan;246:31-42. doi: 10.1016/j.forsciint.2014.10.046. Epub 2014 Nov 11. Forensic Sci Int. 2015. PMID: 25460105 Free article.
Contribution of Atrial Fibrillation to In-Hospital Mortality in Patients With COVID-19.
Spinoni EG, Mennuni M, Rognoni A, Grisafi L, Colombo C, Lio V, Renda G, Foglietta M, Petrilli I, D'Ardes D, Sainaghi PP, Aimaretti G, Bellan M, Castello L, Avanzi GC, Corte FD, Krengli M, Pirisi M, Malerba M, Capponi A, Gallina S, Pierdomenico SD, Cipollone F, Patti G; COVID-UPO Clinical Team†. Spinoni EG, et al. Circ Arrhythm Electrophysiol. 2021 Feb;14(2):e009375. doi: 10.1161/CIRCEP.120.009375. Epub 2021 Feb 16. Circ Arrhythm Electrophysiol. 2021. PMID: 33591815 Free PMC article. No abstract available.
Predictive Value of Echocardiographic Pulmonary to Left Atrial Ratio for In-Hospital Death in Patients with COVID-19.
Renda G, Mennuni MG, Pizzoferrato G, Esposto D, Alberani A, De Vecchi S, Degiovanni A, Giubertoni A, Spinoni EG, Grisafi L, Sagazio E, Ucciferri C, Falasca K, Vecchiet J, Gallina S, Patti G. Renda G, et al. Diagnostics (Basel). 2023 Jan 7;13(2):224. doi: 10.3390/diagnostics13020224. Diagnostics (Basel). 2023. PMID: 36673034 Free PMC article.
Elucidating the role of water in collagen self-assembly by isotopically modulating collagen hydration.
Giubertoni G, Feng L, Klein K, Giannetti G, Rutten L, Choi Y, van der Net A, Castro-Linares G, Caporaletti F, Micha D, Hunger J, Deblais A, Bonn D, Sommerdijk N, Šarić A, Ilie IM, Koenderink GH, Woutersen S. Giubertoni G, et al. Proc Natl Acad Sci U S A. 2024 Mar 12;121(11):e2313162121. doi: 10.1073/pnas.2313162121. Epub 2024 Mar 7. Proc Natl Acad Sci U S A. 2024. PMID: 38451946 Free PMC article.
Surprisingly, collagen assembly occurs ten times faster in D[Formula: see text]O than in H[Formula: see text]O, and collagen in D[Formula: see text]O self-assembles into much thinner fibrils, that form a more inhomogeneous and softer network, with a fourfold reducti …
Surprisingly, collagen assembly occurs ten times faster in D[Formula: see text]O than in H[Formula: see text]O, and collagen in D