Larvicidal and Enzymatic Inhibition Effects of Annona muricata Seed Extract and Main Constituent Annonacin against Aedes aegypti and Aedes albopictus (Diptera: Culicidae)

Alzeir Machado Rodrigues; Antonio Adailson Sousa Silva; Cleonilda Claita Carneiro Pinto; Dayanne Lima Dos Santos; José Claudio Carneiro de Freitas; Victor Emanuel Pessoa Martins; Selene Maia de Morais

doi:10.3390/ph12030112

Larvicidal and Enzymatic Inhibition Effects of Annona muricata Seed Extract and Main Constituent Annonacin against Aedes aegypti and Aedes albopictus (Diptera: Culicidae)

Pharmaceuticals (Basel). 2019 Jul 26;12(3):112. doi: 10.3390/ph12030112.

Authors

Alzeir Machado Rodrigues^{1

2}, Antonio Adailson Sousa Silva³, Cleonilda Claita Carneiro Pinto⁴, Dayanne Lima Dos Santos⁵, José Claudio Carneiro de Freitas⁶, Victor Emanuel Pessoa Martins⁷, Selene Maia de Morais⁸

Affiliations

¹ Departamento de Ensino, Ciências e Formação de Professores, Instituto Federal de Educação, Ciência e Tecnologia do Pará, Avenida Almirante Barroso, 1155, Belém 66093-020, Pará, Brazil. alzeir.rodrigues@ifpa.edu.br.
² Programa de Pós-Graduação em Biotecnologia, RENORBIO, Universidade Estadual do Ceará, Avenida Doutor Silas Munguba, 1700, Fortaleza 60741-000, Ceará, Brazil. alzeir.rodrigues@ifpa.edu.br.
³ Programa de Pós-Graduação em Farmacologia, Universidade Federal do Ceará, Rua Coronel Nunes de Melo, 1127, Fortaleza 60430-275, Ceará, Brazil.
⁴ Programa de Pós-Graduação em Biotecnologia, RENORBIO, Universidade Estadual do Ceará, Avenida Doutor Silas Munguba, 1700, Fortaleza 60741-000, Ceará, Brazil.
⁵ Laboratório de Química de Produtos Naturais, Universidade Estadual do Ceará, Avenida Doutor Silas Munguba, 1700, Fortaleza 60741-000, Ceará, Brazil.
⁶ Vertrauen Diagnosis, Avenida Washington Soares, 655, Fortaleza 60810-000, Ceará, Brazil.
⁷ Instituto de Ciências Exatas e da Natureza, Universidade da Integração Internacional da Lusofonia Afro-Brasileira (Unilab), Campus das Auroras, Rua José Franco de Oliveira s/n, Redenção 62790-970, Ceará, Brazil.
⁸ Departamento de Química, Universidade Estadual do Ceará, Avenida Doutor Silas Munguba, 1700, Fortaleza 60741-000, Ceará, Brazil. selenemaiademorais@gmail.com.

Abstract

The mosquitoes Aedes aegypti and Aedes albopictus are vectors of arboviruses that cause dengue, zika and chikungunya. Bioactive compounds from plants are environmentally sustainable alternatives to control these vectors and thus the arboviruses transmitted by them. The present study evaluated the larvicidal activity of an acetogenin-rich fraction (ACERF) and its main constituent annonacin obtained from Annona muricata seeds on Ae. aegypti and Ae. albopictus. The larvicidal assays were performed using different concentrations to calculate the LC₅₀ and LC₉₀ values observed 24 h after exposure to the treatment. Annonacin was more active against Ae. aegypti (LC₅₀ 2.65 μg·mL^-¹) in comparison with Ae. albopictus (LC₅₀ 8.34 μg·mL^-¹). In contrast, the acetogenin-rich fraction was more active against Ae. albopictus (LC₅₀ 3.41 μg·mL^-¹) than Ae. aegypti (LC₅₀ 12.41 μg·mL^-¹). ACERF and annonacin treated larvae of Ae. aegypti and Ae. albopictus showed significant differences in the inhibition of their metabolic enzymes when compared to untreated larvae. The results demonstrate the relevant larvicidal action of the acetogenin-rich fraction and annonacin showing the potential to develop new products for the control of Ae. aegypti and Ae. albopictus.

Keywords: Aedes; annonacin; botanicals; larvicidal.