Pesticide residues on Ginkgo leaves are a safety concern. A fast and reliable method was developed for the analysis of 81 pesticides on Ginkgo leaves, which are pharmaceutically valuable. The method consists of a modified Quick, Easy, Cheap, Effective, Rugged, and Safe sample preparation method and GC/MS detection. Five sorbents (graphitized carbon black, primary secondary amine, amino, alumina, and Florisil) and two solvents (toluene and hexane) were optimized for the cleanup procedure. The LOD and LOQ ranged from 0.05 to 17.6 and 0.16 to 58.8 microg/kg, respectively. A majority of the pesticides (86-94%) gave satisfactory recoveries (70-110%), and 1-6% of the substances gave recoveries higher than 110% of the spiked concentration (n = 5) at 0.02, 0.05, and 0.1 mg/kg. Less than 5% of the substances showed recoveries lower than 70%. The method was proven to be repeatable with RSDs lower than 20%.