Biological Compositions of Canine Amniotic Membrane and Its Extracts and the Investigation of Corneal Wound Healing Efficacy In Vitro

Chompunut Permkam; Gunnaporn Suriyaphol; Sujin Sirisawadi; Nalinee Tuntivanich

doi:10.3390/vetsci9050227

Biological Compositions of Canine Amniotic Membrane and Its Extracts and the Investigation of Corneal Wound Healing Efficacy In Vitro

Vet Sci. 2022 May 9;9(5):227. doi: 10.3390/vetsci9050227.

Authors

Chompunut Permkam¹, Gunnaporn Suriyaphol², Sujin Sirisawadi², Nalinee Tuntivanich³

Affiliations

¹ Veterinary Bioscience Program, Department of Veterinary Anatomy, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
² Biochemistry Unit, Department of Veterinary Physiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
³ Department of Veterinary Surgery, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.

Abstract

The usage of canine amniotic membrane (cAM) is mainly of interest in veterinary ophthalmology. Topical formulations of cAM could deliver the beneficial properties of cAM without the need for surgical intervention. The present study aimed to investigate biological compositions of cAM and its extracts, including their corneal wound healing efficacy. In this study, canine amniotic membrane extract (cAME) and lyophilized canine amniotic membrane extract (cAMX) were developed. Bioactive molecules related to corneal wound healing, including hepatocyte growth factor, tissue inhibitor of metalloproteinase-1 and -2, Thrombospondin-1 and Interleukin-1 receptor antagonist were studied at both gene and protein expression levels. Cell viability and wound healing assays were investigated for the possibility of cAME and cAMX as topical applications. The results demonstrated that all of the relevant genes and proteins were detected in cAM, cAME and cAMX. Both cAME and cAMX showed wound healing properties in vitro and cAME at 1.0 mg/mL concentration appeared to have the best healing efficacy. In conclusion, cAME and cAMX generated for topical use provided promising results in the healing of corneal defects.

Keywords: amniotic membrane; canine; cornea; extract; lyophilized; wound healing.

Grants and funding

This research was funded by “the 100th Anniversary Chulalongkorn University Fund for Doctoral Scholarship” and “the 90th Anniversary of Chulalongkorn University Fund (Ratchadaphiseksomphot Endowment Fund)”, Grant number GCUGR1125623068D. Both scholarships were provided by Graduate School of Chulalongkorn University, Bangkok, Thailand.