Quantitative Nuclear Magnetic Resonance Spectroscopy Based on PULCON Methodology: Application to Quantification of Invaluable Marine Toxin, Okadaic Acid

Ryuichi Watanabe; Chika Sugai; Taichi Yamazaki; Ryoji Matsushima; Hajime Uchida; Masahiro Matsumiya; Akiko Takatsu; Toshiyuki Suzuki

doi:10.3390/toxins8100294

Quantitative Nuclear Magnetic Resonance Spectroscopy Based on PULCON Methodology: Application to Quantification of Invaluable Marine Toxin, Okadaic Acid

Toxins (Basel). 2016 Oct 13;8(10):294. doi: 10.3390/toxins8100294.

Authors

Ryuichi Watanabe¹, Chika Sugai², Taichi Yamazaki³, Ryoji Matsushima⁴, Hajime Uchida⁵, Masahiro Matsumiya⁶, Akiko Takatsu⁷, Toshiyuki Suzuki⁸

Affiliations

¹ National Research Institute of Fisheries Science, 2-12-4 Fukuura, Kanazawa, Yokohama 236-8648, Japan. rwatanabe@affrc.go.jp.
² Department of Marine Science and Resource, Nihon University, Kameino, Fujisawa 252-0880, Japan. world_wide_love_0511@yahoo.co.jp.
³ National Institute of Advanced Industrial Science and Technology, Tsukuba Chuo 3, 1-1-1 Umezono, Tsukuba 305-8563, Japan. t-yamazaki@aist.go.jp.
⁴ National Research Institute of Fisheries Science, 2-12-4 Fukuura, Kanazawa, Yokohama 236-8648, Japan. matsur@affrc.go.jp.
⁵ National Research Institute of Fisheries Science, 2-12-4 Fukuura, Kanazawa, Yokohama 236-8648, Japan. huchida@affrc.go.jp.
⁶ Department of Marine Science and Resource, Nihon University, Kameino, Fujisawa 252-0880, Japan. matsumiya@brs.nihon-u.ac.jp.
⁷ National Institute of Advanced Industrial Science and Technology, Tsukuba Chuo 3, 1-1-1 Umezono, Tsukuba 305-8563, Japan. akiko-takatsu@aist.go.jp.
⁸ National Research Institute of Fisheries Science, 2-12-4 Fukuura, Kanazawa, Yokohama 236-8648, Japan. tsuzuki@affrc.go.jp.

Abstract

ERETIC2 (Electronic Reference To access In vivo Concentrations 2) based on PULCON (Pulse Length-based Concentration determination) methodology is a quantitative NMR (qNMR) using an external standard. The performance of the PULCON method was assessed using maleic acid (MA). Quantification of the diarrhetic shellfish toxin and okadaic acid by PULCON was successfully consistent with that obtained by a conventional internal standard method, demonstrating that the PULCON method is useful for the quantification of invaluable marine toxins without any contaminations by an internal standard.

Keywords: ERETIC2; PULCON; external standard; okadaic acid; qNMR; quantitative NMR.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Magnetic Resonance Spectroscopy / methods
Maleates / analysis
Marine Toxins / analysis*
Okadaic Acid / analysis*

Substances

Maleates
Marine Toxins
Okadaic Acid
maleic acid