Analyzing the radiation spectra of impurity ions is a widely applied diagnostic scheme for plasma ion temperature and rotation measurements on tokamaks. High precision wavelength calibration is a prerequisite for the accurate measurement of plasma parameters, especially for plasma rotation. Furthermore, the sparseness or absence of the standard spectral lines brings calibration challenges due to the narrow wavelength range. A precise wavelength calibration method is demonstrated in which the comb-like spectra generated by the Fabry-Pérot etalon can lock a series of fixed peaks as reference points in a wide wavelength range. The equal frequency intervals of the comb-like spectra are further corrected using several characteristic neon lines of known wavelengths. The experimental results indicate that the wavelength accuracy obtained by this calibration method is less than 0.005 nm, which corresponds to a rotation speed of 2.3 km/s in the toroidal direction for the beam emission spectroscopy spectrometer installed on the experimental advanced superconducting tokamak. Taking the O V(650.024 nm, n = 4 → 3) line as an example, the maximum difference in the oxygen ion rotation velocity is 3.8 km/s for the absolute rotation of ∼25 km/s, when compared with the calibration results of a standard lamp.