A G-protein-coupled chemokine receptor: A putative insertion site for a multi-pathogen recombinant capripoxvirus vaccine strategy

Catherine Cêtre-Sossah; Simon Dickmu; Olivier Kwiatek; Emmanuel Albina

doi:10.1016/j.jim.2017.05.007

A G-protein-coupled chemokine receptor: A putative insertion site for a multi-pathogen recombinant capripoxvirus vaccine strategy

J Immunol Methods. 2017 Sep:448:112-115. doi: 10.1016/j.jim.2017.05.007. Epub 2017 May 31.

Authors

Catherine Cêtre-Sossah¹, Simon Dickmu², Olivier Kwiatek³, Emmanuel Albina⁴

Affiliations

¹ CIRAD UMR ASTRE, F-97490 Sainte Clotilde, France; INRA, UMR1309 CMAEE, F-34398 Montpellier, France. Electronic address: catherine.cetre-sossah@cirad.fr.
² Laboratoire National Vétérinaire, PO Box 503, Garoua, Cameroon.
³ INRA, UMR1309 CMAEE, F-34398 Montpellier, France; CIRAD UMR ASTRE, F-34398 Montpellier, France.
⁴ INRA, UMR1309 CMAEE, F-34398 Montpellier, France; CIRAD UMR ASTRE, F-97170 Petit-Bourg, France.

PMID: 28576653
DOI: 10.1016/j.jim.2017.05.007

Abstract

Capripoxviruses (CaPVs) have been shown to be ideal viral vectors for the development of recombinant multivalent vaccines to enable delivery of immunogenic genes from ruminant pathogens. So far, the viral thymidine kinase (TK) gene is the only gene used to generate recombinants. A putative non-essential gene encoding a G-protein-coupled chemokine receptor subfamily homologue (GPCR) was targeted as an additional insertion site. Peste des petits ruminants (PPR) was chosen as a disease model. A new recombinant CaPV expressing the viral attachment hemagglutinin (H) of the PPR virus (PPRV) in the GPCR insertion site (rKS1-HPPR-GPCR) was generated in the backbone North African isolate KS1 strain of lumpy skin disease virus (LSDV). Comparison with the recombinant CaPV expressing the H of PPRV in the TK gene (rKS1-HPPR-TK) shown to induce protection against both PPR and LSD in both sheep and goats was assessed. The suitability of the GPCR gene to be a putative additional insertion site in the CaPV genome is evaluated and discussed.

Keywords: Capripoxvirus; Goats; New insertion site; Vaccine.

MeSH terms

Animals
Antibodies, Viral / blood
Capripoxvirus / genetics*
Cattle
Chlorocebus aethiops
Genetic Vectors*
Goats
Hemagglutinins, Viral / administration & dosage
Hemagglutinins, Viral / genetics
Hemagglutinins, Viral / immunology
Injections, Subcutaneous
Lumpy Skin Disease / genetics
Lumpy Skin Disease / immunology
Lumpy Skin Disease / prevention & control*
Lumpy Skin Disease / virology
Lumpy skin disease virus / genetics*
Lumpy skin disease virus / immunology
Mutagenesis, Insertional*
Peste-des-Petits-Ruminants / genetics
Peste-des-Petits-Ruminants / immunology
Peste-des-Petits-Ruminants / prevention & control*
Peste-des-Petits-Ruminants / virology
Peste-des-petits-ruminants virus / genetics*
Peste-des-petits-ruminants virus / immunology
Receptors, Chemokine / administration & dosage
Receptors, Chemokine / genetics*
Receptors, Chemokine / immunology
Receptors, G-Protein-Coupled / administration & dosage
Receptors, G-Protein-Coupled / genetics*
Receptors, G-Protein-Coupled / immunology
Vaccines, Synthetic / administration & dosage
Vaccines, Synthetic / genetics
Vaccines, Synthetic / immunology
Vero Cells
Viral Vaccines / administration & dosage
Viral Vaccines / genetics*
Viral Vaccines / immunology
Virus Cultivation

Substances

Antibodies, Viral
Hemagglutinins, Viral
Receptors, Chemokine
Receptors, G-Protein-Coupled
Vaccines, Synthetic
Viral Vaccines