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The coenzyme specificity of Candida tenuis xylose reductase (AKR2B5) explored by site-directed mutagenesis and X-ray crystallography.
Petschacher B, Leitgeb S, Kavanagh KL, Wilson DK, Nidetzky B. Petschacher B, et al. Biochem J. 2005 Jan 1;385(Pt 1):75-83. doi: 10.1042/BJ20040363. Biochem J. 2005. PMID: 15320875 Free PMC article.
Purified single-site mutants K274R (Lys274-->Arg), K274M, K274G, S275A, N276D, R280H and the double mutant K274R-N276D were characterized by steady-state kinetic analysis of enzymic D-xylose reductions with NADH and NADPH at 25 degrees C (pH 7.0). The results reveal bet …
Purified single-site mutants K274R (Lys274-->Arg), K274M, K274G, S275A, N276D, R280H and the double mutant K274R-N276D were characterized …
Xylose reductase from the Basidiomycete fungus Cryptococcus flavus: purification, steady-state kinetic characterization, and detailed analysis of the substrate binding pocket using structure-activity relationships.
Mayr P, Petschacher B, Nidetzky B. Mayr P, et al. J Biochem. 2003 Apr;133(4):553-62. doi: 10.1093/jb/mvg071. J Biochem. 2003. PMID: 12761304 Free article.
Steady-state kinetic parameters for the reaction, D-xylose + NADPH + H(+)<--> xylitol + NADP(+), have been obtained at pH 7.0 and 25 degrees C. The catalytic efficiency for reduction of D-xylose is 150 times that for oxidation of xylitol. ...Total transition state st …
Steady-state kinetic parameters for the reaction, D-xylose + NADPH + H(+)<--> xylitol + NADP(+), have been obtained at pH 7.0 and 25 d …
Altering the coenzyme preference of xylose reductase to favor utilization of NADH enhances ethanol yield from xylose in a metabolically engineered strain of Saccharomyces cerevisiae.
Petschacher B, Nidetzky B. Petschacher B, et al. Microb Cell Fact. 2008 Mar 17;7:9. doi: 10.1186/1475-2859-7-9. Microb Cell Fact. 2008. PMID: 18346277 Free PMC article.
RESULTS: An isogenic pair of yeast strains was derived from S. cerevisiae Cen.PK 113-7D through chromosomal integration of a three-gene cassette that carried a single copy for C. tenuis XR in wild-type or double mutant form, XDH from Galactocandida mastotermitis, and the e …
RESULTS: An isogenic pair of yeast strains was derived from S. cerevisiae Cen.PK 113-7D through chromosomal integration of a three-gene cass …
Fermentation of mixed glucose-xylose substrates by engineered strains of Saccharomyces cerevisiae: role of the coenzyme specificity of xylose reductase, and effect of glucose on xylose utilization.
Krahulec S, Petschacher B, Wallner M, Longus K, Klimacek M, Nidetzky B. Krahulec S, et al. Microb Cell Fact. 2010 Mar 10;9:16. doi: 10.1186/1475-2859-9-16. Microb Cell Fact. 2010. PMID: 20219100 Free PMC article.
We also carried out a comprehensive investigation on the currently unclear role of coenzyme utilization, NADPH compared to NADH, for xylose reduction during co-fermentation of glucose and xylose. RESULTS: BP10001 and BP000, expressing C. tenuis xylose reductase in NADPH-pr …
We also carried out a comprehensive investigation on the currently unclear role of coenzyme utilization, NADPH compared to NADH, for xylose …