Establishment of a TNFRSF11B knock-out human induced pluripotent stem cell line (KSCBi002-B-2) via CRISPR/Cas9 system

Jujin Jeong; Dahye Lee; Byeong-Cheol Park; Tae-Hoon Lee; Sang-Wook Park

doi:10.1016/j.scr.2023.103167

Establishment of a TNFRSF11B knock-out human induced pluripotent stem cell line (KSCBi002-B-2) via CRISPR/Cas9 system

Stem Cell Res. 2023 Sep:71:103167. doi: 10.1016/j.scr.2023.103167. Epub 2023 Jul 14.

Authors

Jujin Jeong¹, Dahye Lee¹, Byeong-Cheol Park¹, Tae-Hoon Lee², Sang-Wook Park³

Affiliations

¹ Department of Oral Biochemistry, School of Dentistry, Chonnam National University, Gwangju 61186, South Korea.
² Department of Oral Biochemistry, School of Dentistry, Chonnam National University, Gwangju 61186, South Korea. Electronic address: thlee83@jnu.ac.kr.
³ Department of Oral Biochemistry, School of Dentistry, Chonnam National University, Gwangju 61186, South Korea. Electronic address: swpark@jnu.ac.kr.

PMID: 37481965
DOI: 10.1016/j.scr.2023.103167

Abstract

A TNFRSF11B (TNF Receptor Superfamily Member 11b) gene encodes a soluble decoy receptor, osteoprotegerin (OPG), which has a key role in repressing osteoclast differentiation. In this report, we generated a biallelic knock-out hiPSC line for the TNFRSF11B gene via CRISPR/Cas9. When TNFRSF11B Knock-out hiPSCs were differentiated into mesenchymal progenitor cells (MPCs), the expression level of OPG was significantly decreased compared to normal hiPSC-derived MPCs. This knock-out hiPSCs will provide a chance to study Paget disease of bone 5 (juvenile Paget disease).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Cas Systems / genetics
Humans
Induced Pluripotent Stem Cells* / metabolism
Osteitis Deformans* / genetics
Osteitis Deformans* / metabolism
Osteoprotegerin / genetics
Osteoprotegerin / metabolism

Substances

Osteoprotegerin
TNFRSF11B protein, human