We produced the first daily gridded meteorological dataset at a 1-km spatial resolution across Serbia for 2000-2019, named MeteoSerbia1km. The dataset consists of five daily variables: maximum, minimum and mean temperature, mean sea-level pressure, and total precipitation. In addition to daily summaries, we produced monthly and annual summaries, and daily, monthly, and annual long-term means. Daily gridded data were interpolated using the Random Forest Spatial Interpolation methodology, based on using the nearest observations and distances to them as spatial covariates, together with environmental covariates to make a random forest model. The accuracy of the MeteoSerbia1km daily dataset was assessed using nested 5-fold leave-location-out cross-validation. All temperature variables and sea-level pressure showed high accuracy, although accuracy was lower for total precipitation, due to the discontinuity in its spatial distribution. MeteoSerbia1km was also compared with the E-OBS dataset with a coarser resolution: both datasets showed similar coarse-scale patterns for all daily meteorological variables, except for total precipitation. As a result of its high resolution, MeteoSerbia1km is suitable for further environmental analyses.